Hyperbaric oxygen treatment on keloid tumor immune gene expression
Open Access
- 13 September 2021
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Chinese Medical Journal
- Vol. 134 (18), 2205-2213
- https://doi.org/10.1097/cm9.0000000000001780
Abstract
Background: Hyperbaric oxygen treatment (HBOT) has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids. To explore the mechanism of the effect of HBOT on keloids, tumor immune gene expression and immune cell infiltration were studied in this work. Methods: From February 2021 to April 2021, HBOT was carried out on keloid patients four times before surgery. Keloid tissue samples were collected and divided into an HBOT group (keloid tissue with HBOT before surgery [HK] group, n = 6) and a non-HBOT group (K group, n = 6). Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit. Data were mined with R package. The differentially expressed genes between the groups were compared. Hub genes between the groups were determined and verified with Quantitative Real-time PCR. Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry (IHC). Results: Inflammatory cell infiltration was reduced in the HBOT group. There were 178 upregulated genes and 217 downregulated genes. Ten hub genes were identified, including Integrin Subunit Alpha M (ITGAM), interleukin (IL)-4, IL-6, IL-2, Protein Tyrosine Phosphatase Receptor Type C (PTPRC), CD86, transforming growth factor (TGF), CD80, CTLA4, and IL-10. CD80, ITGAM, IL-4, and PTPRC with significantly downregulated expression were identified. IL-10 and IL-2 were upregulated in the HK group but without a significant difference. Infiltration differences of CD8 lymphocyte T cells, CD4 lymphocyte T-activated memory cells, and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis. Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification. Conclusion: HBOT affected tumor gene expression and immune cell infiltration in keloids. CD4 lymphocyte T cell, especially activated memory CD4+T, might be the key regulatory immune cell, and its related gene expression needs further study.Keywords
This publication has 27 references indexed in Scilit:
- Treg‐enriched CD4+ T cells attenuate collagen synthesis in keloid fibroblastsExperimental Dermatology, 2014
- Prevention of Autoimmune Diabetes and Induction of β-Cell Proliferation in NOD Mice by Hyperbaric Oxygen TherapyDiabetes, 2012
- Tumor-Like Stem Cells Derived from Human Keloid Are Governed by the Inflammatory Niche Driven by IL-17/IL-6 AxisPLOS ONE, 2009
- Hypertrophic Scars and Keloids—A Review of Their Pathophysiology, Risk Factors, and Therapeutic ManagementDermatologic Surgery, 2009
- α-Chain phosphorylation of the human leukocyte CD11b/CD18 (Mac-1) integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasationBlood, 2006
- Hyperbaric oxygen protects from sepsis mortality via an interleukin-10–dependent mechanism*Critical Care Medicine, 2006
- Genome Scans Provide Evidence for Keloid Susceptibility Loci on Chromosomes 2q23 and 7p11Journal of Investigative Dermatology, 2004
- CD45: A Critical Regulator of Signaling Thresholds in Immune CellsAnnual Review of Immunology, 2003
- Clinical Genetics of Familial KeloidsArchives of Dermatology, 2001
- General Immune Reactivity in Keloid PatientsPlastic and Reconstructive Surgery, 1984