Integrated management of Meloidogyne incognita on tomato using combinations of abamectin, Purpureocillium lilacinum, rhizobacteria, and botanicals compared with nematicide
Open Access
- 16 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Egyptian Journal of Biological Pest Control
- Vol. 31 (1), 1-10
- https://doi.org/10.1186/s41938-021-00438-x
Abstract
Background Acceptable alternative eco-friendly tools in the present study were tested to control the root-knot nematode, Meloidogyne incognita, on greenhouse-cultivated vegetables. The nematicidal effect of rhizobacteria (Pseudomonas and Serratia), egg parasitic fungus (Purpureocillium lilacinum), abamectin (Streptomyces avermitilis), and 3 botanicals (colocynth, Citrullus colocynthis; moringa, Moringa oleifera; marigold, Tagetes erecta L.) singly or in combination was tested against M. incognita, in comparison with emamectin benzoate. Results In vitro treatments revealed that egg hatching and juvenile mortality were influenced by the type of bioagents, plant species of botanicals, and exposure time. All the tested bioagents and botanicals displayed nematicidal potential via their ovicidal and larvicidal action on egg hatching and J2 mortality of M. incognita. Three and 5 days post-treatment, abamectin and emamectin benzoate were more effective than P. lilacinum, Serratia and Pseudomonas, and C. colocynthis in inhibiting egg hatching: 96.31 and 94.88%; 95.79 and 94.05%; 94.11 and 94.46%; 85.54 and 87.28%; 88.87 and 84.30%, respectively. On the other hand, after 10 days, P. lilacinum gave the highest inhibition percentage (99.00%), followed by abamectin (89.25%). However, the difference was insignificant compared with the inhibition percentage of rhizobacteria, Serratia and Pseudomonas (88.69%; p ≤ 0.05). Moreover, juvenile mortality was 100.0, 96.80, and 91.60% after 10 days of treatment, respectively. However, botanicals showed a lower effect on egg hatching and juvenile mortality. Under greenhouse conditions, potential antagonism towards M. incognita by application the mixture of biocontrol agents and botanicals was more effective in controlling M. incognita than single treatments. Conclusions The combination of abamectin and/or emamectin benzoate with P. lilacinum and rhizobacteria was the most effective against M. incognita, followed by rhizobacteria and P. lilacinum, not only in decreasing galls and reproduction of M. incognita but also in increasing plant growth of tomato parameters than the control. The application of various bioagents including abamectin might be a potential antagonism strategy against phytonematodes in protected agricultural areas.Keywords
This publication has 23 references indexed in Scilit:
- Bio-efficacy of some leaf extracts on the inhibition of egg hatching and mortality ofMeloidogyne incognitaArchives of Phytopathology and Plant Protection, 2013
- Top 10 plant‐parasitic nematodes in molecular plant pathologyMolecular Plant Pathology, 2013
- Nematotoxic Phenolic Compounds from Melia azedarach Against Meloidogyne incognitaJournal of Agricultural and Food Chemistry, 2012
- Nematicidal Activity of (E,E)-2,4-Decadienal and (E)-2-Decenal from Ailanthus altissima against Meloidogyne javanicaJournal of Agricultural and Food Chemistry, 2012
- The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodesCrop Protection, 2008
- Studies on the management of root-knot nematode,Meloidogyne incognita-wilt fungus,Fusarium oxysporumdisease complex of green gram,Vigna radiatacv ML-1108Journal of Zhejiang University SCIENCE, 2005
- Antifungal activity in vitro of Aloe vera pulp and liquid fraction against plant pathogenic fungiIndustrial Crops and Products, 2005
- Effects of Paecilomyces lilacinus protease and chitinase on the eggshell structures and hatching of Meloidogyne javanica juvenilesBiological Control, 2004
- Compatibility ofSteinernema feltiae(Nematoda: Steinernematidae) with Pesticides and Plant Growth Regulators Used in Glasshouse Plant ProductionBiocontrol Science and Technology, 2003
- Infection of Meloidogyne javanica by Paecilomyces lilacinusNematology, 1999