Stoppage of bleeding from damaged blood vessels is hemostasis, an essential factor to be checked before any surgical process. Hence blood test is a routine test for hospital prior to surgeries. The aim of this study is to assess the blood groups distribution and to check gender based relationship among blood groups, bleeding and clotting time. The study was conducted in the department of pharmacy, BZU. The available detail reports of 122 students of 1st year of Pharm.D were analyzed in aspects of age, sex, blood groups, bleeding time and clotting time. The blood group were determined with the standard antisera, Duke Method and Capillary tube method were used to find out bleeding time and clotting time respectively. Finally all the parameters were compared and analyzed statistically. In the present study it is found that B blood group is more prevalent in both the sexes than O, A and AB. Clotting time is found to be significantly higher in O and B blood groups in females and males respectively, whereas Bleeding time was found to be prolonged in maximum number of group AB in females and blood group O in males but the difference was statistically insignificant. In females, bleeding and clotting time are slightly on higher side than in males, may be because of hormonal differences. Various cardiovascular and gastrointestinal diseases are associated with the blood groups, so people can take preventive measures according to their blood groups.