Pathological characteristics and bcl11a gene analysis in molecular subtypes of breast cancer

Abstract

Background: Breast cancer is the most frequent cause of cancer-related deaths among women worldwide. Molecular classification and the analysis of gene expression have been playing a pivotal role in deciding treatment therapy. The aim of study was to identify molecular surrogate subtypes of breast cancer by immunohistochemistry and Dual In Situ Hybridization, analysis of BCL11A gene expression was performed in molecular subtypes by RT-PCR and assessed the correlation between molecular subtypes and clinic-pathological characteristics. Materials and methods: Cross-sectional description study, tumor specimens from 138 patients were stained with Hematoxylin and Eosin to diagnosis, histopathological classification, stained with immunohistochemical markers ER, PR, HER2, Ki67 and Dual In Situ Hybridization (DISH) to classify molecular subtypes breast cancer. In which, RNA of 20 samples were extraction and performed by RT-PCR. Results: A significant association between breast carcinoma subtypes with histologic type (p=0.022) and grade (p=0.021). BCL11A was significantly down regulated in all molecular breast cancer subtypes especially in triple negative group. Conclusion: This study may help to understand pathological characteristics and the gene expression of difference breast cancer subtypes, contributing to the successful identification of therapeutic targets for breast cancer, especially triple negative group. Key words: Invasive breast carcinoma, molecular subtypes, histologic grade, Dual In Situ Hybridization (DISH), qRT-PCR