Phage Display Technology, Phage Display System, Antibody Library, Prospects and Challenges
Open Access
- 1 January 2021
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Advances in Microbiology
- Vol. 11 (03), 181-189
- https://doi.org/10.4236/aim.2021.113013
Abstract
Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool. Through panning, a protein or peptide with high affinity and selectivity to the target is obtained. Antibody phage display has become the first and most widely used in vitro screening technology. Phage display derivatives play an important role in the diagnosis and treatment of diseases. This article reviews the phage display system of phage display technology, the size and classification of antibody libraries and their applications, and discusses the application prospects and challenges of phage display technology. This thesis lays the foundation for the theoretical and experimental research of bacteriophages.Keywords
This publication has 22 references indexed in Scilit:
- Phage Display-based Strategies for Cloning and Optimization of Monoclonal Antibodies Directed against Human PathogensInternational Journal of Molecular Sciences, 2012
- Phagemid Vectors for Phage Display: Properties, Characteristics and ConstructionJournal of Molecular Biology, 2012
- Prediction of Aggregation Prone Regions of Therapeutic ProteinsThe Journal of Physical Chemistry B, 2010
- Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotidesJournal of Molecular Biology, 2000
- Making Antibodies by Phage Display TechnologyAnnual Review of Immunology, 1994
- By-passing immunisation: Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitroJournal of Molecular Biology, 1992
- Phage antibodies: filamentous phage displaying antibody variable domainsNature, 1990
- Immune Network TheoryImmunological Reviews, 1989
- Canonical structures for the hypervariable regions of immunoglobulinsJournal of Molecular Biology, 1987
- Somatic generation of antibody diversityNature, 1983