Kadmiyuma Maruz Kalan Ratlarda Oral Melatonin Uygulamasının Oksidatif Stres ve Bağırsak Mikroflorası Üzerine Etkisi

Abstract

The aim of the present study was to evaluate the effects of oral melatonin treatment on oxidative stress and intestinal microflora in rats exposed to chronic cadmium. Healthy 32 adult male albino Wistar rats were randomly divided into four groups as control (C; n=8), cadmium (Cd; n=8), melatonin (Mel, n=8), cadmium + melatonin (Cd+Mel; n=8). Mel (100mg/kg) was orally administered 5 times (Mel and Cd+Mel), and CdCl2 (2mg/day) 3 times a week for 4 weeks to rats (Cd and Cd+Mel). After the treatments, serum TAS, TOS levels as well as plasma ALT, AST, GGT, T.pro, Alb and BUN values were measured. Intestinal contents were aseptically collected, and Enterobacteriaceae, Lactococcus spp. and Lactobacillus spp. counts were performed. As a result, serum TOS levels were defined higher in Cd group than other groups (P <0.05). Lactococcus spp. counts decreased as 0.63 log in Cd group compared to C in small intestine, however it increased as 1.15 log in Cd + M group. In conclusion, Cd+Mel chelate was found benefical for intestinal microflora due to suppressed the Enterobacteriaceae growth, however ameliorated the Cd induced oxidative stress and Lactobacillus spp./Lactococcus spp. rates in the different part of the intestine.