Identification of VPA1327 (vopT) as a Novel Genetic Marker for Detecting Pathogenic Vibrio parahaemolyticus
Open Access
- 30 June 2018
- journal article
- research article
- Published by Journal of Pure and Applied Microbiology in Journal of Pure and Applied Microbiology
- Vol. 12 (2), 429-438
- https://doi.org/10.22207/jpam.12.2.01
Abstract
Vibrio parahaemolyticus is a Gram-negative bacterium that is ubiquitous in marine and estuarine environments. The pathogenic strains can cause foodborne gastroenteritis especially when raw or undercooked seafood contaminated with pathogenic V. parahaemolyticus are consumed. This study aims to obtain a novel species-specific genetic marker using comparative genomics and to investigate their prevalence in both clinical and seafood isolates of V. parahaemolyticus. VPA1327 (vopT) was identified as a unique gene of this organism, but it was reported present in only 33.3% of V. parahaemolyticus (n = 72 isolates). Interestingly, vopT was found exclusively in clinical isolates. The combination PCR analysis was used to determine the presence of three genes (tdh, trh, and vopT) in 36 clinical isolates. Four genotypes were classified namely, tdh(+)trh(-)vopT(+) (24 isolates, 66.7%), tdh(+)trh(+)vopT(-)(2 isolates, 5.6%), tdh(-)trh(+)vopT(-) (2 isolates, 5.6%) and tdh(-)trh(-)vopT(-)(8 isolates, 22.2%). Furthermore, all vopT(+) isolates were tdh(+) isolates, and none of them coexist with trh(+) isolates. This study has provided strong evidence to support vopT as an additional virulence marker for the detection of V. parahaemolyticus with the pathogenic potential to cause human disease.Keywords
This publication has 31 references indexed in Scilit:
- Biochemical, Serological, and Virulence Characterization of Clinical and Oyster Vibrio parahaemolyticus IsolatesJournal of Clinical Microbiology, 2012
- Distribution of type III secretion systems in Vibrio parahaemolyticus from the northern Gulf of MexicoJournal of Applied Microbiology, 2010
- Species-specific PCR detection of the food-borne pathogen Vibrio parahaemolyticus using the irgB gene identified by comparative genomic analysisFEMS Microbiology Letters, 2010
- Identification and Characterization of a Novel Type III Secretion System in trh -Positive Vibrio parahaemolyticus Strain TH3996 Reveal Genetic Lineage and Diversity of Pathogenic Machinery beyond the Species LevelInfection and Immunity, 2009
- Pathogenetic characterization of Vibrio parahaemolyticus isolates from clinical and seafood sourcesInternational Journal of Food Microbiology, 2008
- Identification of Vibrio Isolates by a Multiplex PCR Assay and rpoB Sequence DeterminationJournal of Clinical Microbiology, 2007
- Global Dissemination of Vibrio parahaemolyticus Serotype O3:K6 and Its SerovariantsClinical Microbiology Reviews, 2007
- Vibrio parahaemolyticus Disruption of Epithelial Cell Tight Junctions Occurs Independently of Toxin ProductionInfection and Immunity, 2005
- The ADP Ribosyltransferase Domain of Pseudomonas aeruginosa ExoT Contributes to Its Biological ActivitiesInfection and Immunity, 2004
- The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysinsReviews in Medical Microbiology, 1993