Production and Characterization of New Fibrinolytic Protease from Mucor subtillissimus UCP 1262 in Solid-State Fermentation
Open Access
- 1 January 2015
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Advances in Enzyme Research
- Vol. 03 (03), 81-91
- https://doi.org/10.4236/aer.2015.33009
Abstract
Fibrinolytic enzymes have received attention regarding their medicinal potential for thrombolytic diseases, a leading cause of morbidity and mortality worldwide. Various natural enzymes purified from animal, plant and microbial sources have been extensively studied. The aim of this work was to produce fibrinolytic protease by solid state fermentation using agro industrial substrates. Rhizopus arrhizus var. arrhizus UCP 1295 and Mucor subtillissimus UCP 1262 filamentous fungi species isolated from soil of Caatinga-PE, Brasil, were used as producer microorganisms. Wheat bran was shown to be the best substrate for the production of the enzyme and by using a 23 full factorial design the main effects and interactions of the quantity of the substrate wheat bran, moisture and temperature on the fibrinolytic enzyme production and protease were evaluated. The best results for fibrinolytic and protease activities, 144.58 U/mL and 48.33 U/mL, respectively, were obtained with Mucor subtillissimus UCP 1262 using as culture medium 3 g wheat bran, 50% moisture at a temperature of 25°C for 72 hours. The optimum temperature for the produced enzyme was 45°C and most of its original activity was retained after being subjected to 80°C for 120 min. The protease activity was enhanced by K+, Ca+ and Mn+; but with Cu+ there was an inhibition. The specificity to chromogenic substrate and the inhibition by PMSF indicates that it is a chymotrypsin-like serine protease. Presented results suggest that this enzyme produced by solid-state fermentation is an interesting alternative as a candidate for thrombolytic therapy.Keywords
This publication has 45 references indexed in Scilit:
- Purification and biochemical properties of a fibrinolytic enzyme from Bacillus subtilis-fermented red beanFood Chemistry, 2012
- Uniform culture in solid-state fermentation with fungi and its efficient enzyme productionJournal of Bioscience and Bioengineering, 2011
- Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007New Biotechnology, 2011
- A novel fibrinolytic protease from Streptomyces sp. CS684Process Biochemistry, 2010
- Purification and characterization of fibrinolytic metalloprotease from Perenniporia fraxinea myceliaMycological Research, 2008
- Caracterização de compostos nitrogenados presentes em farelos fermentados em estado sólidoFood Science and Technology, 2007
- Production of alkaline protease by Penicillium sp. under SSF conditions and its application to soy protein hydrolysisProcess Biochemistry, 2004
- BIOPROCESS DEVELOPMENT FOR SERINE ALKALINE PROTEASE PRODUCTION: A REVIEWReviews in Chemical Engineering, 2001
- Purification and properties of an extracellular endoglucanase from Myceliophthora thermophila D-14 (ATCC 48104)Journal of General Microbiology, 1990
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976