Soil samples and granulodensimetric fractions isolated from them ((silt particle size less than 1 µm, light fraction (LF) with a density of less than 2 g/cm3 and a fraction of the residues) of ordinary chernozem from experimental fields of the Kamennaya Steppe agricultural landscape of the Voronezh region were studied. The main differences between the variants of the experiment are introducing or stopping mineral fertilizers, and in the aftereffect of irrigation (13 years). There is an increase in the content of LF when using mineral fertilizers, as well as a change in the composition of sizedensity fractionations, expressed in different content of C and N and hydrophobic-hydrophilic components of humus substances (HS) of soils, silt and LF. The irrigation had practically no effect on the hydrophobichydrophilic composition of the initial soils, while the relative content of the hydrophilic components of the silt fraction of the soil increased, and the hydrophilic composition of HS LF decreased. The use of mineral fertilizers led to a significant variation in the degree of hydrophilicity of HS in silt and LF with a smaller change of this indicator for the HS of the soil as a whole. The abolition of fertilizers application, on the contrary, affected the hydrophobichydrophilic composition of the soil as a whole, and the composition of the soil silt and LF. The increase in the proportion of hydrophilic components in the composition of soil HS, as well as in the composition of HS silt and LF occurred simultaneously with an increase in the hydrophobicity of the surface of the solid phase of soil and with an increase in the carbon content in the soil, which indicates the stability of the system as a whole. Since the physical fractions of soils reacted more intensively to changes in the agrogenic load, compared with native soil samples, and changes in their qualitative composition were traced in two versions of the experiment, monitoring of hydrophobic-hydrophilic components of soil and soil granulodensimetric fractions is advisable to monitor and diagnose soil changes during agricultural use.