Distinct Co-occurrence Relationships and Assembly Processes of Active Methane-Oxidizing Bacterial Communities Between Paddy and Natural Wetlands of Northeast China
Open Access
- 26 January 2022
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Microbiology
- Vol. 13, 809074
- https://doi.org/10.3389/fmicb.2022.809074
Abstract
Studies of methane-oxidizing bacteria are updating our views of their composition and function in paddy and natural wetlands. However, few studies have characterized differences in the methane-oxidizing bacterial communities between paddy and natural wetlands. Here, we conducted a 13C stable isotope-probing experiment and high-throughput sequencing to determine the structure profiling, co-occurrence relationships, and assembly processes of methanotrophic communities in four wetlands of Northeast China. There was a clear difference in community structure between paddy and natural wetlands. LEfSe analyses revealed that Methylobacter, FWs, and Methylosinus were enriched in natural wetlands, while Methylosarcina were prevailing in paddy, all identified as indicative methanotrophs. We observed distinct co-occurrence relationships between paddy and natural wetlands: more robust and complex connections in natural wetlands than paddy wetlands. Furthermore, the relative importance of stochastic processes was greater than that of deterministic processes, as stochastic processes explained >50% of the variation in communities. These results demonstrated that the co-occurrence relationships and assembly processes of active methanotrophic communities in paddy and natural wetlands were distinct. Overall, the results of this study enhance our understanding of the communities of methane-oxidizing bacteria in paddy and natural wetlands of Northeast China.Funding Information
- Chinese Academy of Sciences (XDA28020202)
- National Natural Science Foundation of China (91951109)
This publication has 68 references indexed in Scilit:
- Advances, challenges and a developing synthesis of ecological community assembly theoryPhilosophical Transactions B, 2011
- Metagenomic biomarker discovery and explanationGenome Biology, 2011
- Stable Isotope Probing Analysis of the Diversity and Activity of Methanotrophic Bacteria in Soils from the Canadian High ArcticApplied and Environmental Microbiology, 2010
- Search and clustering orders of magnitude faster than BLASTBioinformatics, 2010
- Different Atmospheric Methane-Oxidizing Communities in European Beech and Norway Spruce SoilsApplied and Environmental Microbiology, 2010
- Picante: R tools for integrating phylogenies and ecologyBioinformatics, 2010
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- The global methane cycle: recent advances in understanding the microbial processes involvedEnvironmental Microbiology Reports, 2009
- Molecular Ecology Techniques for the Study of Aerobic MethanotrophsApplied and Environmental Microbiology, 2008
- NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genesNucleic Acids Research, 2006