Effects of Mono-2-ethylhexyl Phthalate on the Neural Transmission of PNs in Drosophila Antennal Lobe
- 30 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Neurotoxicity Research
- Vol. 39 (5), 1430-1439
- https://doi.org/10.1007/s12640-021-00386-2
Abstract
Long-term exposure to different types of chemicals is hazardous to human health. Di(2-ethylhexyl) phthalate (DEHP) could exert pleiotropic deleterious effects on nervous systems. Mono(2-ethylhexyl) phthalate (MEHP), as one of the most toxic metabolites of DEHP, may have similar effects on nervous systems. However, no effects of MEHP on neural circuits have been reported. To uncover the regulation of MEHP on neural transmission, the functional changes of neural excitability and synaptic plasticity of projection neurons (PNs) have been assessed. In the current study, we recorded the action potentials (APs), stimulate action potentials (sti-APs), mini excitement postsynaptic current (mEPSC), calcium currents, and sodium currents from PNs of isolated whole brain of Drosophila model utilizing patch clamp recordings. We found that MEHP-300 (at the concentration of 300 μM), but not MHEP-100 (at the concentration of 100 μM), significantly decreased the frequency and amplitude of APs. Besides, the amplitude and anti-amplitude of sti-APs were reduced with the application of MEHP-300. Meanwhile, MEHP-300 reduced the frequency of mEPSC, but not the amplitude. Furthermore, MEHP-300 reduced the peak current densities of sodium and calcium channels. Therefore, our results indicated that MEHP could alter the neural excitability and synaptic plasticity of PNs by inhibiting the ion channels activities, revealing the potential modulation of MEHP on neural transmission of PNs.Keywords
Funding Information
- Natural Science Foundation of Chongqing (cstc2020jcyj-msxmX0706)
- Youth Project of Science and Technology Research Program of Chongqing Education Commission of China (KJQN202000412)
This publication has 44 references indexed in Scilit:
- Developmental Exposure to Di-(2-ethylhexyl) Phthalate Induces Cerebellar Granule Cell Apoptosis via the PI3K/AKT Signaling PathwayExperimental Neurobiology, 2018
- DEHP toxicity on vision, neuromuscular junction, and courtship behaviors of DrosophilaEnvironmental Pollution, 2018
- Impact of Di-2-Ethylhexyl Phthalate Metabolites on Male Reproductive Function: a Systematic Review of Human EvidenceCurrent Environmental Health Reports, 2018
- Inhibition of oxidative stress in cholinergic projection neurons fully rescues aging-associated olfactory circuit degeneration in DrosophilaeLife, 2018
- Electrophysiological analysis of synaptic transmission in DrosophilaWIREs Developmental Biology, 2017
- First Steps toward Harmonized Human Biomonitoring in Europe: Demonstration Project to Perform Human Biomonitoring on a European ScaleEnvironmental Health Perspectives, 2015
- Involvement of hemeoxygenase-1 in di(2-ethylhexyl) phthalate (DEHP)-induced apoptosis of Neuro-2a cellsThe Journal of Toxicological Sciences, 2014
- Voltage‐gated sodium channels at 60: structure, function and pathophysiologyJournal Of Physiology-London, 2012
- Mono-(2-ethylhexyl) phthalate impairs neurodevelopment: Inhibition of proliferation and promotion of differentiation in PC12 cellsToxicology Letters, 2011
- Hepatocarcinogenic Potential of Di(2-Ethylhexyl)phthalate in Rodents and its Implications on Human RiskCritical Reviews in Toxicology, 1996