Current Immunoassay Methods for the Rapid Detection of Aflatoxin in Milk and Dairy Products
- 23 August 2017
- journal article
- Published by Wiley in Comprehensive Reviews in Food Science and Food Safety
- Vol. 16 (5), 808-820
- https://doi.org/10.1111/1541-4337.12287
Abstract
The presence of mycotoxins in foodstuff causes serious health problems to consumers and economically affects the food industry. Among the mycotoxins, aflatoxins are very toxic and highly carcinogenic contaminants which affect the safety of many foods, and therefore endanger human health. Aflatoxin M1 (AFM1) found in milk results from the biotransformation of aflatoxin B1. Many efforts have been made to control the source of AFM1 from farmers to dairy product companies. However, AFM1 escapes ordinary methods of food treatment such as cooking, sterilization, and freezing, hence it appears in milk and dairy products. The presence of high levels of AFM1 constitutes an alarming threat as milk and dairy products contain essential nutrients for human health, especially for infants and children. For this reason, there is a pressing need for developing a fast and reliable screening method for detecting trace aflatoxins in food. Several analytical methods based on high-performance liquid chromatography (HPLC) and mass spectroscopy have been used for aflatoxin detection; however, they are expensive, time-consuming, and require many skills. Recently, immunoassay methods, including enzyme-linked immunosorbent assay (ELISA), immunosensors, and lateral flow immunoassay (LFIA), have been preferred for food analysis because of their improved qualities such as high sensitivity, simplicity, and capability of onsite monitoring. This paper reviews the new developments and applications of immunoassays for the rapid detection of AFM1 in milk.Keywords
This publication has 104 references indexed in Scilit:
- Mycotoxins: Occurrence, toxicology, and exposure assessmentFood and Chemical Toxicology, 2013
- Highly sensitive FRET-based fluorescence immunoassay for aflatoxin B1 using cadmium telluride quantum dotsMicrochimica Acta, 2013
- Optimization of a lateral flow immunoassay for the ultrasensitive detection of aflatoxin M1 in milkAnalytica Chimica Acta, 2013
- Impact of mycotoxins on humans and animalsJournal of Saudi Chemical Society, 2011
- Contamination of UHT milk by aflatoxin M1 in IranFood Control, 2010
- Detection of aflatoxin M1 in milk products from China by ELISA using monoclonal antibodiesFood Control, 2009
- Immunoaffinity pre-concentration combined with on-column visual detection as a tool for rapid aflatoxin M1 screening in milkFood Control, 2009
- Analytical methods for determination of mycotoxins: A reviewAnalytica Chimica Acta, 2009
- Isolation and affinity maturation of hapten-specific antibodiesBiotechnology Advances, 2007
- Flow injection immunoassays: A reviewMicrochimica Acta, 1998