Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection
Published: 16 February 2021
in PLoS ONE
PLoS ONE , Volume 16; https://doi.org/10.1371/journal.pone.0246302
Abstract: Background: Two automatable in-house protocols for high-troughput RNA extraction from nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated.Methods: One hundred forty one SARS-CoV-2 positive samples were collected during a period of 10-days. In-house protocols were based on extraction with magnetic beads and designed to be used with either the Opentrons OT-2 (OT-2in-house) liquid handling robot or the MagMAXTMExpress-96 system (MMin-house). Both protocols were tested in parallel with a commercial kit that uses the MagMAXTMsystem (MMkit). Nucleic acid extraction efficiencies were calculated from a SARS-CoV-2 DNA positive control.Results: No significant differences were found between both in-house protocols and the commercial kit in their performance to detect positive samples. The MMkitwas the most efficient although the MMin-housepresented, in average, lower Cts than the other two. In-house protocols allowed to save between 350€ and 400€ for every 96 extracted samples compared to the commercial kit.Conclusion: The protocols described harness the use of easily available reagents and an open-source liquid handling system and are suitable for SARS-CoV-2 detection in high throughput facilities.
Keywords: Nucleic acids / SARS CoV 2 / RNA extraction / Elution / Viral load / Ethanol / Virus testing / COVID 19
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