Adsorption of Biomineralization Protein Mms6 on Magnetite (Fe3O4) Nanoparticles
Open Access
- 16 May 2022
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 23 (10), 5554
- https://doi.org/10.3390/ijms23105554
Abstract
Biomineralization is an elaborate process that controls the deposition of inorganic materials in living organisms with the aid of associated proteins. Magnetotactic bacteria mineralize magnetite (Fe3O4) nanoparticles with finely tuned morphologies in their cells. Mms6, a magnetosome membrane specific (Mms) protein isolated from the surfaces of bacterial magnetite nanoparticles, plays an important role in regulating the magnetite crystal morphology. Although the binding ability of Mms6 to magnetite nanoparticles has been speculated, the interactions between Mms6 and magnetite crystals have not been elucidated thus far. Here, we show a direct adsorption ability of Mms6 on magnetite nanoparticles in vitro. An adsorption isotherm indicates that Mms6 has a high adsorption affinity (Kd = 9.52 µM) to magnetite nanoparticles. In addition, Mms6 also demonstrated adsorption on other inorganic nanoparticles such as titanium oxide, zinc oxide, and hydroxyapatite. Therefore, Mms6 can potentially be utilized for the bioconjugation of functional proteins to inorganic material surfaces to modulate inorganic nanoparticles for biomedical and medicinal applications.Funding Information
- Japan Society for the Promotion of Science (16H02421, 18H01794)
- United States Army Research Office (W911NF-20-1-0201)
This publication has 57 references indexed in Scilit:
- Integrated Self-Assembly of the Mms6 Magnetosome Protein to Form an Iron-Responsive StructureInternational Journal of Molecular Sciences, 2013
- MMS6 Protein Regulates Crystal Morphology during Nano-sized Magnetite Biomineralization in VivoOnline Journal of Public Health Informatics, 2011
- Analysis of an ultra hard magnetic biomineral in chiton radular teethMaterials Today, 2010
- Controlled formation of magnetite crystal by partial oxidation of ferrous hydroxide in the presence of recombinant magnetotactic bacterial protein Mms6Biomaterials, 2007
- The structure, dynamics, and energetics of protein adsorption—lessons learned from adsorption of statherin to hydroxyapatiteMagnetic Resonance in Chemistry, 2007
- Protein‐Mediated Synthesis of Uniform Superparamagnetic Magnetite NanocrystalsAdvanced Functional Materials, 2007
- Enzymatic Synthesis of Layered Titanium Phosphates at Low Temperature and Neutral pH by Cell-Surface Display of Silicatein-αJournal of the American Chemical Society, 2005
- Bone recognition mechanism of porcine osteocalcin from crystal structureNature, 2003
- Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1Nature Materials, 2003
- Interaction of Mugineic Acid with Synthetically Produced Iron OxidesSoil Science Society of America Journal, 1993