Metataxonomic Analysis of Milk Samples From SARS-CoV-2-Positive and SARS-CoV-2-Negative Women
Open Access
- 18 March 2022
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Nutrition
- Vol. 9, 853576
- https://doi.org/10.3389/fnut.2022.853576
Abstract
Objective: To assess the impact of SARS-CoV-2 viral infection on the metataxonomic profile and its evolution during the first month of lactation. Methods: Milk samples from 37 women with full-term pregnancies and mild SARS-CoV-2 infection and from 63 controls, collected in the first and fifth postpartum weeks, have been analyzed. SARS-CoV-2 RNA was assessed by reverse transcription polymerase chain reaction (RT-PCR) both in cases and controls. After DNA extraction, the V3-V4 hypervariable region of the gene 16S rRNA was amplified and sequenced using the MiSeq system of Illumina. Data were submitted for statistical and bioinformatics analyses after quality control. Results: All the 1st week and 5th week postpartum milk samples were negative for SARS-CoV-2 RNA. Alpha diversity showed no differences between milk samples from the study and control group, and this condition was maintained along the observation time. Analysis of the beta-diversity also indicated that the study and control groups did not show distinct bacterial profiles. Staphyloccus and Streptococcus were the most abundant genera and the only ones that were detected in all the milk samples provided. Disease state (symptomatic or asymptomatic infection) did not affect the metataxonomic profile in breast milk. Conclusion: These results support that in the non-severe SARS-CoV-2 pregnant woman infection the structure of the bacterial population is preserved and does not negatively impact on the human milk microbiota.This publication has 77 references indexed in Scilit:
- Vertical mother–neonate transfer of maternal gut bacteria via breastfeedingEnvironmental Microbiology, 2013
- Human milk: a source of more life than we imagineBeneficial Microbes, 2013
- The SILVA ribosomal RNA gene database project: improved data processing and web-based toolsNucleic Acids Research, 2012
- Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studiesNucleic Acids Research, 2012
- Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human MilkPLOS ONE, 2011
- Inhibition of Human Immunodeficiency Virus Type 1 by Lactic Acid Bacteria from Human BreastmilkBreastfeeding Medicine, 2010
- Respiratory syncytial virus-induced dysregulation of expression of a mucosal β-defensin augments colonization of the upper airway by non-typeableHaemophilus influenzaeCellular Microbiology, 2009
- Assessment of the bacterial diversity of human colostrum and screening of staphylococcal and enterococcal populations for potential virulence factorsResearch in Microbiology, 2008
- Staphylococcus epidermidis: A differential trait of the fecal microbiota of breast-fed infantsBMC Microbiology, 2008
- The Composition of the Faecal Microflora in Breastfed and Bottle Fed Infants from Birth to Eight WeeksActa Paediatrica, 1985