Raman Computational and Experimental Studies of Dopamine Detection
Open Access
- 28 September 2017
- journal article
- research article
- Published by MDPI AG in Biosensors
- Vol. 7 (4), 43
- https://doi.org/10.3390/bios7040043
Abstract
A combined theoretical and experimental analysis of dopamine (DA) is presented in this work with the objective of achieving more accurate detection and monitoring of this neurotransmitter at very low concentrations, specific to physiological levels. Surface-enhanced Raman spectroscopy on silver nanoparticles was employed for recording DA concentrations as low as 10−11 molar. Quantum chemical density functional calculations were carried out using Gaussian-09 analytical suite software. Relatively good agreement between the simulated and experimentally determined results indicates the presence of different DA molecular forms, such as uncharged DA±, anionic DA−, and dopaminequinone. Disappearance of the strongest bands of dopamine around 750 cm−1 and 790 cm−1, which suggests its adsorption onto the metallic surface, is not only consistent with all of these DA configurations, but also provides additional information about the analyte’s redox process and voltammetric detection. On the other hand, occurrence of the abovementioned Raman lines could indicate the formation of multilayers of DA or its presence in a cationic DA+ form. Thus, through coordinated experiment and theory, valuable insights into changes observed in the vibrational signatures of this important neurotransmitter can be achieved for a better understanding of its detection at physiological levels, which is crucial if further optovoltammetric medical device development is envisioned.Keywords
This publication has 29 references indexed in Scilit:
- Super-resolution Optical Imaging of Single-Molecule SERS Hot SpotsNano Letters, 2010
- Single-molecule and single-nanoparticle SERS: from fundamental mechanisms to biomedical applicationsChemical Society Reviews, 2008
- Neurotransmitters in key neurons of the hypothalamus that regulate feeding behavior and body weightPhysiology & Behavior, 2007
- Proof of Single-Molecule Sensitivity in Surface Enhanced Raman Scattering (SERS) by Means of a Two-Analyte TechniqueThe Journal of Physical Chemistry B, 2006
- Introductory Lecture : Surface enhanced Raman spectroscopy: new materials, concepts, characterization tools, and applicationsFaraday Discussions, 2005
- The ‘chemical’ (electronic) contribution to surface‐enhanced Raman scatteringJournal of Raman Spectroscopy, 2005
- Expectation and Dopamine Release: Mechanism of the Placebo Effect in Parkinson's DiseaseScience, 2001
- Surface-enhanced Raman spectroscopy of the catecholamine neurotransmitters and related compoundsAnalytical Chemistry, 1988
- Development of the Colle-Salvetti correlation-energy formula into a functional of the electron densityPhysical Review B, 1988
- Micro carbon electrode for intracellular voltammetryAnalytical Chemistry, 1986