Screening of Sepsis Biomarkers Based on Bioinformatics Data Analysis
Open Access
- 26 September 2022
- journal article
- research article
- Published by Hindawi Limited in Journal of Healthcare Engineering
- Vol. 2022, 1-10
- https://doi.org/10.1155/2022/6788569
Abstract
Background and objectives. Sepsis is a life-threatening organ dysfunction caused by the imbalance of the bodys response to infection. Delay in sepsis diagnosis has become a primary cause of patient death. This study aims to identify potential biomarkers of sepsis based on bioinformatics data analysis, so as to provide new gene biomarkers for the diagnosis and treatment of sepsis. Methods. Gene expression profiles of GSE13904, GSE26378, GSE26440, GSE65682, and GSE69528 were obtained from the National Center for Biotechnology Information (NCBI). The differentially expressed genes (DEGs) were searched using limma software package. Gene Ontology (GO) functional analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein-protein interaction (PPI) network analysis were performed to elucidate molecular mechanisms of DEGs and screen hub genes. Results. A total of 108 DEGs were identified in the study, of which 67 were upregulated and 41 were downregulated. 15 superlative diagnostic biomarkers (CCL5, CCR7, CD2, CD27, CD274, CD3D, GNLY, GZMA, GZMH, GZMK, IL2RB, IL7R, ITK, KLRB1, and PRF1) for sepsis were identified by bioinformatics analysis. Conclusion. 15 hub genes (CCL5, CCR7, CD2, CD27, CD274, CD3D, GNLY, GZMA, GZMH, GZMK, IL2RB, IL7R, ITK, KLRB1, and PRF1) have been elucidated in this study, and these biomarkers may be helpful in the diagnosis and therapy of patients with sepsis.Keywords
Funding Information
- Natural Science Foundation of Hunan Province (2021JJ31005)
This publication has 72 references indexed in Scilit:
- Involvement of lymphocytes in asthma and allergic diseasesCurrent Opinion in Allergy and Clinical Immunology, 2013
- Drug resistance mechanism of PncA inMycobacterium tuberculosisJournal of Biomolecular Structure and Dynamics, 2013
- clusterProfiler: an R Package for Comparing Biological Themes Among Gene ClustersOMICS: A Journal of Integrative Biology, 2012
- In silico investigation of molecular mechanism of laminopathy caused by a point mutation (R482W) in lamin A/C proteinAmino Acids, 2011
- Selective blockade of interleukin-6 trans-signaling improves survival in a murine polymicrobial sepsis model*Critical Care Medicine, 2011
- Perioperative Gene Expression Analysis for Prediction of Postoperative SepsisClinical Chemistry, 2010
- CCR7 regulates lymphocyte egress and recirculation through body cavitiesJournal of Leukocyte Biology, 2009
- Granzyme H induces apoptosis of target tumor cells characterized by DNA fragmentation and Bid-dependent mitochondrial damageMolecular Immunology, 2008
- Natural killer cell–derived human granzyme H induces an alternative, caspase-independent cell-death programBlood, 2007
- Sepsis and cytokines: current statusBritish Journal of Anaesthesia, 1996