Differential pulse voltammetry and chronoamperometry as analytical tools for epinephrine detection using a tyrosinase-based electrochemical biosensor
Open Access
- 6 September 2022
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in RSC Advances
- Vol. 12 (39), 25342-25353
- https://doi.org/10.1039/d2ra04045j
Abstract
The main goal of the presented study was to design a biosensor-based system for epinephrine (EP) detection using a poly-thiophene derivative and tyrosinase as a biorecognition element. We compared two different electroanalytical techniques to select the most prominent technique for analyzing the neurotransmitter. The prepared biosensor system exhibited good parameters; the differential pulse (DPV) technique presented a wide linear range (1–20 μM and 30–200 μM), with a low detection limit (0.18 nM and 1.03 nM). In the case of chronoamperometry (CA), a high signal-to-noise ratio and lower reproducibility were observed, causing a less broad linear range (10–200 μM) and a higher detection limit (125 nM). Therefore, the DPV technique was used for the calculation of sensitivity (0.0011 μA mM−1 cm−2), stability (49 days), and total surface coverage (4.18 × 10−12 mol cm−2). The biosensor also showed very high selectivity in the presence of common interfering species (i.e. ascorbic acid, uric acid, norepinephrine, dopamine) and was successfully applied for EP determination in a pharmaceutical sample.Keywords
Funding Information
- Politechnika Wrocławska
- Università degli Studi di Sassari
This publication has 57 references indexed in Scilit:
- Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrineInternational Journal of Nanomedicine, 2013
- An Improved Chronoamperometry for Electrochemical Real-Time PCR MeasurementAdvanced Materials Research, 2011
- Biosensor based on platinum nanoparticles dispersed in ionic liquid and laccase for determination of adrenalineSensors and Actuators B: Chemical, 2009
- Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexesActa Biomaterialia, 2008
- Electrochemical biosensors: Towards point-of-care cancer diagnosticsBiosensors and Bioelectronics, 2006
- Kinetics of self-assembled layer formation on ironElectrochimica Acta, 2002
- Determination of β-HCG using amperometric immunosensors based on a conducting immunocompositeAnalytica Chimica Acta, 1999
- Cerebrospinal Fluid Epinephrine in Alzheimer's Disease and Normal AgingNeuropsychopharmacology, 1998
- Determination of 5-hydroxytryptamine (serotonin) and related indoles by flow injection analysis with acidic potassium permanganate chemiluminescence detectionAnalytica Chimica Acta, 1998
- Serotonin in body fluids: Characterization of human plasmatic and cerebrospinal fluid pools by means of a new HPLC methodLife Sciences, 1985