A validated method for coumarin quantification in Meliloti herba and its ethanolic extracts using micellar thin-layer chromatography
Open Access
- 12 October 2021
- journal article
- Published by Heighten Science Publications Corporation in Annals of Advances in Chemistry
- Vol. 5 (1), 013-018
- https://doi.org/10.29328/journal.aac.1001024
Abstract
A micellar thin-layer chromatography method for the quantitative determination and validation of coumarin in Meliloti herba and its ethanolic extracts was developed and validated. For achieving good determination, the mobile phase of 5 x 10-4 mol/L Tween-80 in a mixture propanol-2 – water (5:95 v/v) was used. Densitometric determination was carried out at 275 nm. The calibration curve was linear in the range of 0.1-2.5 μg per band. The proposed method is simple, rapid, precise and accurate; replacing hazardous solvents by greener ones correspond to the modern requirements in “Green chemistry” concepts. The obtained data can be used for the routine analysis of coumarin in medical plant and extracts.Keywords
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