Degraded polysaccharides from Porphyra haitanensis: purification, physico-chemical properties, antioxidant and immunomodulatory activities
- 13 September 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Glycoconjugate Journal
- Vol. 38 (5), 573-583
- https://doi.org/10.1007/s10719-021-10009-9
Abstract
To explore effect of the structural properties of porphyra haitanensis polysaccharide on its biological activity, degraded porphyra polysaccharides were separated and purified by Cellulose DEAE-52 and Sephadex G-100 chromatography, obtaining three purified components (P1, P2 and P3). All the three components were sulfate polysaccharides containing the repeating units of → 3) β-D-galactose (1 → 4) 3,6-anhydro-α-L-galactose (1 →, and → 3) β-D-galactose (1 → 4) α-L-galactose-6-S (1 →, and → 3) 6-O-methyl-β-D-galactose (1 → 4) 3,6-anhydro-α-L-galactose (1 →. The molecular weight of the three fractions was measured to be 300.3, 130.4 and 115.1 kDa, respectively. Their antioxidant activity was investigated by the determination of the free radical scavenging effect and ferric reducing power. It was found that P1, P2 and P3 possessed marked antioxidant activity. It was also found that they appreciably enhanced the proliferation, phagocytic ability and nitric oxide secretion in RAW264.7 cells. Lower molecular weight and higher sulfate content were beneficial to bioactivities of P. haitanensis polysaccharides. Overall, P2 and P3 possess superior immuno-modulatory activity to that of P1 and PHP. Thus, the current work will provide the basis for the better utilization of P. haitanensis to develop the related functional foods. Graphical abstractKeywords
Funding Information
- The National Key R&D Program of China (2017YFD0400203)
- Science and Technology Department of Zhejiang Province of China (2018C02048)
This publication has 43 references indexed in Scilit:
- Degradation of sulfated polysaccharides from Enteromorpha prolifera and their antioxidant activitiesCarbohydrate Polymers, 2013
- Degradation and the antioxidant activity of polysaccharide from Enteromorpha linzaCarbohydrate Polymers, 2013
- Stimulation of phagocytosis by sulforaphaneBiochemical and Biophysical Research Communications, 2011
- Sulfated modification, characterization and structure–antioxidant relationships of Artemisia sphaerocephala polysaccharidesCarbohydrate Polymers, 2010
- Isolation and structural characterization of a novel polysaccharide prepared from Arca subcrenata LischkeJournal of Bioscience and Bioengineering, 2007
- Structure and antiviral activity of sulfated fucans from Stoechospermum marginatumPhytochemistry, 2006
- Bifunctional effects of fucoidan on the expression of inducible nitric oxide synthaseBiochemical and Biophysical Research Communications, 2006
- Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitroInternational Journal of Biological Macromolecules, 2005
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976