The Combination of Estradiol and N-Acetylcysteine Reduces Ischemia–Reperfusion Injuries of Mice Autografted Ovarian Tissue
- 1 February 2024
- journal article
- research article
- Published by Mary Ann Liebert Inc in Biopreservation and Biobanking
- Vol. 22 (1), 29-37
- https://doi.org/10.1089/bio.2022.0184
Abstract
Ischemia–reperfusion injuries are important issues after ovarian tissue transplantation (OTT). Our study examined the effects of N-acetylcysteine (NAC) and estradiol (E2) on mouse ovarian autografts. Mice (6–8 weeks) were divided into ovarian autograft as follows: Control: fresh OTT; Sham: cryopreserved/warmed OTT; NAC: cryopreserved/warmed OTT with NAC treatment; E2: cryopreserved/warmed OTT with E2 treatment; NAC+E2: cryopreserved/warmed OTT with the treatment of NAC and E2. In all groups, grafts were harvested on days 2, 7, and 28 after transplantation to evaluate histological parameters, inflammation relative to genes expression, and oxidative status. Histological analysis showed that NAC, E2, and a combination of NAC+E2 significantly increased the primordial, preantral, and antral follicular number. When NAC was used, it significantly reduced the expression of Tnf-α and Fgf-2, whereas it increased Il-1β, Il-6, and Vegf expression levels. The levels of Il-6, Fgf-2, and VEGF were dramatically increased in the E2-treated group. The combination of NAC and E2 significantly increased levels of Il-1β, Il-6, Fgf-2, and Vegf. NAC and E2 alone or in combination significantly increased total antioxidant capacity but did not affect the superoxide dismutase and glutathione peroxidase activities. In conclusion, after transplantation, NAC and E2 alone or in combination, could improve follicular development and angiogenesis as well as decline inflammation and ovarian oxidative damage.Keywords
This publication has 35 references indexed in Scilit:
- Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in RatsBalkan Medical Journal, 2013
- Aquaporin-1 and Aquaporin-3 Expressions in the Intervertebral Disc of Rats with AgingBalkan Medical Journal, 2012
- Assessment of canine ovaries autografted to various body sitesTheriogenology, 2012
- Hypoxia-Induced Angiogenesis: Good and EvilGenes & Cancer, 2011
- N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusionNephrology Dialysis Transplantation, 2006
- Methods for quantifying follicular numbers within the mouse ovaryReproduction, 2004
- The Effect of Glutathione and N-Acetylcysteine on Lipoperoxidative Damage in Patients with Early Septic ShockAmerican Journal of Respiratory and Critical Care Medicine, 2000
- Vascular endothelial growth factor messenger ribonucleic acid expression in the primate ovary.Endocrinology, 1992
- N-Acetylcysteine and glutathione as inhibitors of tumor necrosis factor productionCellular Immunology, 1992
- Pattern of interleukin 6 gene expression in vivo suggests a role for this cytokine in angiogenesis.Proceedings of the National Academy of Sciences of the United States of America, 1990