Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots
- 26 December 2019
- journal article
- research article
- Published by Brill in Israel Journal of Plant Sciences
- Vol. 67 (1-2), 52-68
- https://doi.org/10.1163/22238980-20191113
Abstract
The present study investigated how auxin concentration and the method of application affected the formation of adventitious roots in microshoots of chestnut (Castanea sativa) and oak (Quercus robur). The activity of two urea derivatives (2, 3-MDPU and 3, 4-MDPU) was also evaluated. Microshoots were derived from basal sprouts of two mature chestnut trees (P1 and P2) and one adult oak genotype (Sainza). In chestnut, rooting percentage was positively affected by auxin in a dose-dependent manner, particularly in shoots treated with the hormone for 24 h. The effect of auxin on rooting also differed depending on the application method. In shoots treated for 24 h, the highest concentration of auxin produced the healthiest rooted plantlets, in terms of the root system and shoot quality. By contrast, in shoots treated by the basal quick-dip method, the shoot quality was best at the lowest auxin concentration. The effect of urea derivatives on the root system depended on the species as well as on the auxin concentration and application period. Use of the MDPUs improved the root system architecture of auxin-treated shoots by promoting lateral root development and triggering the synchronous initiation of root primordia at the base of the shoot. Shoot quality was also improved by MDPUs, which promoted resumption of growth and reduced shoot-tip necrosis.Keywords
This publication has 30 references indexed in Scilit:
- Two-stage culture procedure using thidiazuron for efficient micropropagation of Stevia rebaudiana, an anti-diabetic medicinal herb3 Biotech, 2013
- Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L.Plant Growth Regulation, 2011
- CsSCL1 is differentially regulated upon maturation in chestnut microshoots and is specifically expressed in rooting-competent cellsTree Physiology, 2011
- Regeneration in plants and animals: dedifferentiation, transdifferentiation, or just differentiation?Trends in Cell Biology, 2011
- Somaclonal variation in plants: causes and detection methodsPlant Growth Regulation, 2010
- Adventitious Root Formation: New Insights and PerspectivesPublished by Wiley ,2009
- Reprogramming adult cells during organ regeneration in forest speciesPlant Signaling & Behavior, 2009
- Urea derivatives on the move: cytokinin‐like activity and adventitious rooting enhancement depend on chemical structurePlant Biology, 2009
- Rooting response of shoot cuttings from three peach growth habitsScientia Horticulturae, 2007
- Thidiazuron induced somatic embryogenesis and plant regeneration in Capsicum annuumBiologia plantarum, 2006