Analysis of Cytokines and ATP in Plucked Hair Follicles
- 16 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Bulletin of Experimental Biology and Medicine
- Vol. 170 (3), 299-302
- https://doi.org/10.1007/s10517-021-05055-0
Abstract
The concentrations of ATP, IL-6, and IL-10 were measured in extracts of plucked hair follicles from healthy volunteers (normal values) and patients with androgenetic alopecia and then, ATP, IL-6, and IL-10 content was calculated for each follicle. The resulting values were directly proportional to hair follicle length, except for IL-6. The concentration of extracted ATP correlated with lactate dehydrogenase activity indicating cell damage. In patients with androgenetic alopecia, IL-10 content exceeded the normal values in follicles with a length 2 mm long. The content of IL-6 and IL-10 measured by ELISA was comparable with results of mRNA expression assayed by RT-PCR, which attested to moderate level of gene expression. The content of ATP and IL- 10, but not IL-6 depended on the length of plucked hair follicle and on pathogenetic factors affecting hair growth.Keywords
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