Fishery sector plays a vital role in the socio-economic development of Bangladesh. In the fish economy of Bangladesh, one of the most flourishing traffic activities is fish marketing. This study was aimed to investigate the fish species availability, supply chain, quality loss, price behavior and constrains associated with fish marketing in order to provide better suggestion for efficient fish marketing in Jamalpur district of Bangladesh. Data were collected form Sadar upazila markets by using a structured interview schedule, focus group discussions (FGD) and key informant interviews (KII) with the stakeholders involved in supplying fish from farm to fork, such as- aratdars, retailers, and consumers during 2020-21. A total 78 fish species were found (72 Fresh water and 2 Marine water), among them 20 frequently, 17 less amount, 25 occasionally, and 16 species were rarely available in the market. It was estimated that 72% fish species were sourced from culture fishery whereas 28% were from capture fishery. About 91.5% fishes in Jamalpur markets were supplied locally, whereas rests were from other districts. The post-harvest quality losses of fish in the sampled markets were assessed which indicate that small indigenous species (SIS) spoiled quicker than medium to large sizes fishes due to different factors. The price behavior of the fish market influenced by the demand of the fish species, quality, size, catching sources (culture fishery or capture fishery) and the purchasing time (beginning, middle or last time of market). The study found positive correlation between the market price and fish quality. Several problems of fish market were identified resulting consumer dissatisfaction and financial losses for fish traders. Considering the importance of fish market to the economy, this study suggested to take necessary steps for establishing modern fish market to ensure the adequate quality maintenance of fish with efficient marketing.