Immunomodulatory Effect of Virgin Coconut Oil in Wistar Rats Infected with Staphylococcus aureus
Open Access
- 16 March 2021
- journal article
- research article
- Published by Indonesian Center for Animal Research and Development (ICARD) in Jurnal Ilmu Ternak dan Veteriner
- Vol. 26 (1), 31-38
- https://doi.org/10.14334/jitv.v26i1.2670
Abstract
Virgin coconut oil (VCO) contains bioactive that induce immunity against infectious diseases. This study aim to determine the immunomodulatory effects of VCO based on the activity of superoxide dismutase (SOD), lymphocyte proliferation, and histopathological examination in liver and kidney of rats infected with Staphylococcus aureus. The VCO was given intragastrically to rats with a dose of 250 µL for one week. The rats were infected with S. aureus at 5x102 bacterial cells intraperitoneally. Twenty (20) female Wistar rats of one month old were divided into four groups. The negative control group (C-): without treatment, AV group: infected with S. aureus followed by VCO treatment; VA group: pretreated with VCO followed by S. aureus infection, and positive control group (C+): were infected with S. aureus without VCO. All rats were euthanized and necropsied based on the animal ethic standard. Plasma samples were taken to evaluate SOD activity, and lymphocytes were isolated from the spleen to determine their proliferative ability. Livers and kidneys were collected for a histopathology examination. Results showed that the VA group had the highest SOD activity on the 4th week (41.50 ± 3.56 %) and lymphocyte proliferation (0.3018) compared to all treatments, indicating immunomodulatory effects of VCO. Liver of treatments group showed leucocytes infiltration, no hemorrhages (VA); the hepatocytes with normal cells (VA). Kidney of treatments group showed leucocytes infiltration (AV); normal epithelial glomerulus and tubulus cells, still found hemorrhage (VA). These studies indicated that VCO has a potential role as an immunomodulator, hepatoprotectant, and nephroprotectant.Keywords
This publication has 32 references indexed in Scilit:
- Chronic, low concentration exposure to pharmaceuticals impacts multiple organ systems in zebrafishAquatic Toxicology, 2013
- Abscess Formation and Alpha-Hemolysin Induced Toxicity in a Mouse Model of Staphylococcus aureus Peritoneal InfectionInfection and Immunity, 2012
- Review article: the reversibility of cirrhosisAlimentary Pharmacology & Therapeutics, 2012
- Environmentally Persistent Free Radicals (EPFRs). 1. Generation of Reactive Oxygen Species in Aqueous SolutionsEnvironmental Science & Technology, 2011
- Pathogenesis of Liver FibrosisAnnual review of pathology, 2011
- Staphylococcus aureus Sepsis and Mitochondrial Accrual of the 8-Oxoguanine DNA Glycosylase DNA Repair Enzyme in MiceAmerican Journal of Respiratory and Critical Care Medicine, 2011
- Comparative Validation of a Novel Risk Score for Predicting Bleeding Risk in Anticoagulated Patients With Atrial Fibrillation: The HAS-BLED (Hypertension, Abnormal Renal/Liver Function, Stroke, Bleeding History or Predisposition, Labile INR, Elderly, Drugs/Alcohol Concomitantly) ScoreJournal of Invasive Cardiology, 2011
- Astaxanthin prevents in vitro auto-oxidative injury in human lymphocytesCell Biology and Toxicology, 2010
- Virgin coconut oil supplemented diet increases the antioxidant status in ratsFood Chemistry, 2006
- Antifibrotic Agents for Liver DiseaseAmerican Journal of Transplantation, 2005