Antibiotic Susceptibility and Resistance of Clinical Isolates against Various Antibiotics
Open Access
- 31 December 2022
- journal article
- Published by CrossLinks International Publishers in MARKHOR (The Journal of Zoology)
Abstract
Antibiotics are a vital tool in the treatment of a wide range of bacterial diseases, but their overuse and abuse are leading to bacterial resistance. Culture and sensitivity are the best test to select appropriate antibiotics. Objective: To evaluate the antibiotic susceptibility of clinical isolates to various antibiotics. Methods: Collection of samples was done from patients at the Fatima Memorial Hospital's pathology department in Lahore, Pakistan. Total 1000 clinical isolates were isolated from 1400 samples collected. Conventional culture and biochemical tests were used for the identification of bacteria. Antibacterial activity were assessed by comparing antibiotic susceptibility patterns of Gram positive clinical isolates to 26 commercial antibiotic discs (Amikacin, Amoxycillin, Ampicillin, Augmetin, Cefazolin, Cefepime, Cefixime, Cefotaxime, Cefoxiitin, Cefriaxone, Ceftazidime, Cefuroxime, Cephalothin, Ciprofloxicin, Clindamycin, Gentamycin, Imipenem, Levofloxacin, Linezolid, Meropenem, Nalidixic Acid, Nitrofurantoin, Norfloxacin, Ofloxacin, Rifampicin, and Vancomycin) by using Kirby-Bauer disc diffusion method. Results: A total of 1000 clinical isolates had been identified. Among Gram Positive isolates, the most common pathogen was Staphylococcus aureus 400 (40.0%) and Streptococcus pyogenes 50 (5.0%). Conclusion: There is need to improve the technical facilities to minimize the antibiotic resistance by selecting appropriate antibiotics and proper hand washing recommended.Keywords
This publication has 8 references indexed in Scilit:
- Bacterial Isolates and its Antibiotic Susceptibility Pattern in NICUKathmandu University Medical Journal, 2014
- Emergence of carbapenem-resistant Acinetobacter baumannii in hospitals in PakistanJournal of Medical Microbiology, 2014
- Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococci in Rawalpindi, PakistanBritish Journal of Medicine and Medical Research, 2013
- Fourteen years in resistanceInternational Journal of Antimicrobial Agents, 2012
- Antibiotic resistance and its cost: is it possible to reverse resistance?Nature Reviews Microbiology, 2010
- Device-associated nosocomial infection rates in intensive care units of seven Indian cities. Findings of the International Nosocomial Infection Control Consortium (INICC)Journal of Hospital Infection, 2007
- Relationships between antimicrobial use and antimicrobial resistance in Gram-negative bacteria causing nosocomial infections from 1991–2003 at a university hospital in TaiwanInternational Journal of Antimicrobial Agents, 2005
- Magnesium in cardiovascular and other disordersAmerican Journal of Health-System Pharmacy, 2004