For increasing productivity in poultry, antibiotics are overused. This increased use in antibiotics has raise the prevalence of Multidrug resistant (MDR) bacteria in poultry. Treatment of chicken infected with MDR bacteria is difficult to achieve, thereby increasing treatment cost and productivity cost. MDR bacteria of poultry can also infect humans if they are not handled properly. Thus, the purpose of this study was to find bacteria responsible for infecting chicken and prevalence of MDR bacteria in diseased chicken. Out of total 516 diseased chicken, 212(41.09%) chicken were infected by bacteria. The prevalence of E. coli (63.2%) was high in diseased chicken followed by Salmonella spp. (12.26%), Pseudomonas spp. (5.2%) and, Pasteurella spp. (4.7%). Out of total number of isolates, the prevalence of MDR was 42.5 %. This study also showed that Pasteurella spp. isolates had high MDR with prevalence of 50%. It is thus concluded that there was high prevalence of MDR bacteria among diseased chicken in Chitwan district.