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Structure Prediction and Enzymatic Properties of Phytase phyS

Yueming Li, Hongqing Xu, Jianchun Xu, Ruilian Pang, Bingzheng Xu
Advances in Enzyme Research , Volume 7, pp 57-65; doi:10.4236/aer.2019.74005

Abstract: Phytase is a kind of enzyme that hydrolyzes phytic acid and its salts to produce inositol and phosphoric acid. As a new feed additive, phytase has great potential in animal nutrition and environmental protection. Because of its good stability, large-scale production and high activity, microbial phytase has become a hot spot in industrial application. Here, we reported the predicted structure and enzymatic properties of a phytase from Bacillus subtilis, which was named as phyS. It was clear that the optimal temperature is 35°C, and the optimal pH is 8. Meanwhile, the enzyme activity was kept at above 90% in the range of pH 8 - 9, this result demonstrated that phyS is an alkaline phytase. This study lays a foundation for the extensive application of phyS.
Keywords: phytase / Enzymatic Properties / structural prediction

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