Molecular and Physiological Characterization of Copper-Resistant Bacteria Isolated from Activated Sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia
Open Access
- 1 September 2012
- journal article
- Published by Indonesian Society for Microbiology in Microbiology Indonesia
- Vol. 6 (3), 107-116
- https://doi.org/10.5454/mi.6.3.3
Abstract
Copper resistant bacteria can be isolated from environments where copper levels are abundant from mining, industrial, or agricultural activities. The aim of this work was to study the molecular and physiologicalcharacteristics of indigenous copper resistant bacteria isolated from activated sludge in an industrial wastewatertreatment plant in Surabaya, Indonesia. The bacterial isolates were designated as strains IrC1, IrC2, and IrC4. Phylogenetic analysis based on 16S rDNA sequence analysis identified isolates IrC1, IrC2, and IrC4 as Acinetobacter oleivorans (98.41% similarity), Acinetobacter pitii (97.22% similarity), and Cupriavidus pauculus (96.99 similarity), respectively. The addition of 5 mM of CuSO4 in the medium affected morphological 4 appearance of all isolates to green and undulate margin might be due to the survival mechanism of bacteria by absorbing the copper. This studies indicated that copper resistance mechanism of all isolates was facilitated through the bioaccumulation of copper inside the cell, especially on the membrane fraction and inside the cytoplasm, albeit at a limited amount. It was observed that isolates IrC1, IrC2, and IrC4 were capable of accumulating 137.23 , 364.66 , and 272.07 mg L-1 of copper, respectively from the medium containing 8 mM CuSO4. The capability of isolates IrC1, IrC2, and IrC4 to accumulate copper can be exploited in bioremediation 4 process for removing copper from industrial sewage.Keywords
This publication has 30 references indexed in Scilit:
- Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and BurkholderiaBMC Microbiology, 2011
- The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicityProceedings of the National Academy of Sciences of the United States of America, 2009
- Isolation and characterization of the heavy metal resistant bacteria CCNWRS33-2 isolated from root nodule of Lespedeza cuneata in gold mine tailings in ChinaJournal of Hazardous Materials, 2009
- Characterization of the CopR Regulon of Lactococcus lactis IL1403Journal of Bacteriology, 2008
- Intracellular Copper Does Not Catalyze the Formation of Oxidative DNA Damage in Escherichia coliJournal of Bacteriology, 2007
- Studies on Metal Microbe Interaction of Three Bacterial Isolates From East Calcutta WetlandOnLine Journal of Biological Sciences, 2007
- An Outbreak Due to MultiresistantAcinetobacter baumanniiin a Burn Unit: Risk Factors for Acquisition and ManagementInfection Control & Hospital Epidemiology, 2002
- Sargassum seaweed as biosorbent for heavy metalsWater Research, 2000
- Molecular mechanisms of copper resistance and accumulation in bacteriaFEMS Microbiology Reviews, 1994
- Inducible Plasmid-mediated Copper Resistance in Escherichia coliMicrobiology, 1985