Abstract: Ostrich farming is an important growing industry in Pakistan. Its business and importance is growing day by day. However, prevalence of bacteria is major threat to ostrich industry. Objective: To identify the dominant bacteria in the feces of ostriches. Methods: The ostrich that was kept in captivity at the W.A. Apparel factory provided the fecal samples. The samples were inoculated on EMB for the isolation of E. coli. Antibacterial effect of Moringa oleifera seeds and tea leaves with the use of chloroform as a solvent. The antibacterial activity was tested against E. coli using disc diffusion method. Amoxicillin and erythromycin were used as a control antibiotics. Results: It was noticed that tea extract did not show any antimicrobial activity against E. coli. However, Moringa oleifera seeds were effective against E. coli. Conclusion: It was concluded that Moringa oleifera seeds have the potential to work against E. coli.