Angiotensin-II as a Trigger Factor in the Development of Retinopathy of Prematurity
Open Access
- 27 December 2020
- journal article
- Published by PE Polunina Elizareta Gennadievna in Ophthalmology in Russia
- Vol. 17 (4), 746-751
- https://doi.org/10.18008/1816-5095-2020-4-746-751
Abstract
The multifactorial nature of the retinopathy of prematurity (ROP) pathogenesis, makes the thorough study of the mechanism of pathological retinal neovascularization actual. However recently the attention of scientists has been attracted by the participation of renin-angiotensin system (RAS) in the development of retinal vasoproliferative diseases. Purpose: to study the role of AT-II in the pathogenesis of experimental ROP (EROP) in the original model of the disease. Material and methods. To reproduce EROP Wistar rats (n = 15) were exposed to the oxygen concentration varying from 60 to 15% every 12 hours for 14 days from the first day after birth followed by room air for 7 days. Throughout the experiment, the room maintained a constant temperature (+26 °C) and light regime (12 hours a day, 12 hours a night) modes. Control rats (n = 12) were born and kept under normal oxygen content (21 %). Batches of EROP (n = 5) and control (n = 4) rats were sacrificed on 7, 14 and 21 days. All rats underwent binocular enucleation, after which every eyeball was opened on the limb, the cornea and lens were removed with the remains of a persistent vascular bag and a hyaloid artery. Retinas were isolated, homogenized and stored at -20 °C. Angiotensin-II (AT-II) in homogenates was measured using the IFA kit. Results. On the 7th day of the experiment, the level of AT-II in the retina of the experimental group rats was 0.19 ± 0.02 pg/mg protein that was significantly higher than in controls (0.12 ± 0.01 pg/mg protein). On the 14th and 21st days concentrations of AT-II in EROP and control groups had no significant difference. Conclusion. On the 7th day of the experiment, i.e. at the period corresponding to the existence of avascular retinal zones in both groups concentration of AT-II in the retinas of rats with EROP was significantly higher than in controls. This fact indicate the role of this proangiogenic factor in the induction of pathological neovascularization in ROP. Possible prognostic function of this parameter during the period before ROP manifestation has undoubted practical significance.Keywords
This publication has 10 references indexed in Scilit:
- Incidence and Early Course of Retinopathy of PrematurityJAMA Ophthalmology, 2018
- THE CUTTING EDGE OF RETINOPATHY OF PREMATURITY CARERetina, 2017
- PATHOMORPHOLOGICAL FEATURES OF THE DEVELOPMENT OF EXPERIMENTAL RETINOPATHY OF PREMATURITYInternational Journal of Applied and Fundamental Research (Международный журнал прикладных и фундаментальных исследований), 2017
- Involvement of Renin-Angiotensin System in Retinopathy of Prematurity - A Possible Target for Therapeutic InterventionPLOS ONE, 2016
- Angiotensin type‐1 receptor inhibition is neuroprotective to amacrine cells in a rat model of retinopathy of prematurityJournal of Comparative Neurology, 2009
- The Renin-Angiotensin System and the Developing Retinal VasculatureInvestigative Ophthalmology & Visual Science, 2005
- The Renin-Angiotensin System Influences Ocular Endothelial Cell Proliferation in DiabetesThe American Journal of Pathology, 2003
- Angiotensin converting enzyme inhibition reduces retinal overexpression of vascular endothelial growth factor and hyperpermeability in experimental diabetesDiabetologia, 2000
- Effect of lisinopril on progression of retinopathy in normotensive people with type 1 diabetesThe Lancet, 1998
- Demonstration of renin mRNA, angiotensinogen mRNA, and angiotensin converting enzyme mRNA expression in the human eye: evidence for an intraocular renin-angiotensin system.British Journal of Ophthalmology, 1996