The present study was conducted on the Kolar River, a tributary of the Narmada River. The fish diversity of the Kolar River in the Sehore district of Madhya Pradesh, Central India, is represented in this view. It was investigated from February 2021 to June 2022. The Kolar River is a vital source of water for the people who live in nearby villages, primarily for domestic use. In the community, fishing for a living and food is a common practise. The study's goal was to investigate the Kolar River's water quality and fish fauna. During the investigation, six sampling stations were chosen for the collection of data: Near reservoir, Lawakhadi, Veerpur, Near old bridge, Dana baba temple, Amdoh, and Jholiyapur.The study discovered 25 fish species from eight orders (Cypriniformes, Siluriformes, Perciformes, Osteoglossiformes, Synbranchiformes, Clupeiformes, and Beloniformes). Cypriniformes are the dominant order of fishes in this area (Kolar), with the highest number of ten species recorded. The recorded fishes were found to be widely distributed and abundant in the river.