Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions
Open Access
- 1 January 2014
- journal article
- Published by Scientific Research Publishing, Inc. in Open Journal of Applied Biosensor
- Vol. 03 (01), 1-7
- https://doi.org/10.4236/ojab.2014.31001
Abstract
Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn2+ in aqueous solution. The biosensor designed was able to determine Zn2+ in the range of 0.35 - 12 mg/L with a detection limit of 7.5 μg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.Keywords
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