Species-specific formation of paraspeckles in intestinal epithelium revealed by characterization of NEAT1 in naked mole-rat
Open Access
- 2 June 2022
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 28 (8), 1128-1143
- https://doi.org/10.1261/rna.079135.122
Abstract
Paraspeckles are mammalian-specific nuclear bodies built on the long noncoding RNA NEAT1_2. The molecular mechanisms of paraspeckle formation have been mainly studied using human or mouse cells, and it is not known if the same molecular components are involved in the formation of paraspeckles in other mammalian species. We thus investigated the expression pattern of NEAT1_2 in naked mole-rats (nNEAT1_2), which exhibit extreme longevity and lower susceptibility to cancer. In the intestine, nNEAT1_2 is widely expressed along the entire intestinal epithelium, which is different from the expression of mNeat1_2 that is restricted to the cells of the distal tip in mice. Notably, the expression of FUS, a FET family RNA binding protein, essential for the formation of paraspeckles both in humans and mice, was absent in the distal part of the intestinal epithelium in naked mole-rats. Instead, mRNAs of other FET family proteins EWSR1 and TAF15 were expressed in the distal region. Exogenous expression of these proteins in Fus-deficient murine embryonic fibroblast cells rescued the formation of paraspeckles. These observations suggest that nNEAT1_2 recruits a different set of RNA binding proteins in a cell type-specific manner during the formation of paraspeckles in different organisms.Keywords
Funding Information
- Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science/Ministry of Education, Culture, Sports, Science and Technology (21H05274, 21K19246, 17H03604, 21H02392, 21H05143, 19K06469)
- Grant-in-Aid for Early-Career Scientists from JSPS (21K15012)
- PRESTO Program (1159399)
- JST FOREST Program (MJFR216C)
This publication has 47 references indexed in Scilit:
- A triple helix stabilizes the 3′ ends of long noncoding RNAs that lack poly(A) tailsGenes & Development, 2012
- Landscape of transcription in human cellsNature, 2012
- The lncRNA Malat1 Is Dispensable for Mouse Development but Its Transcription Plays a cis-Regulatory Role in the AdultCell Reports, 2012
- Malat1 is not an essential component of nuclear speckles in miceRNA, 2012
- Paraspeckles are subpopulation-specific nuclear bodies that are not essential in miceThe Journal of cell biology, 2011
- Highly Ordered Spatial Organization of the Structural Long Noncoding NEAT1 RNAs within Paraspeckle Nuclear BodiesMolecular Biology of the Cell, 2010
- The Nuclear-Retained Noncoding RNA MALAT1 Regulates Alternative Splicing by Modulating SR Splicing Factor PhosphorylationMolecular Cell, 2010
- MENε/β noncoding RNAs are essential for structural integrity of nuclear paraspecklesProceedings of the National Academy of Sciences of the United States of America, 2009
- MEN ε/β nuclear-retained non-coding RNAs are up-regulated upon muscle differentiation and are essential components of paraspecklesGenome Research, 2008
- 3′ End Processing of a Long Nuclear-Retained Noncoding RNA Yields a tRNA-like Cytoplasmic RNACell, 2008