Design and application of a portable luminometer for bioluminescence detection
- 17 January 2020
- journal article
- research article
- Published by Optica Publishing Group in Applied Optics
- Vol. 59 (3), 801-810
- https://doi.org/10.1364/ao.59.000801
Abstract
The silicon photomultiplier (SiPM) for low light detection has many advantages when compared to existing photon counting detectors, such as high sensitivity, low cost, robustness, and compact hardware. To facilitate the use of SiPM as a portable, field deployable device, an electrical circuit was designed consisting of an amplifier, comparator, and microcontroller. In addition, a 3D printing was used to create a portable cradle for housing the SiPM. To evaluate its detection ability, a laser experiment and bioluminescent experiments, including Pseudomonas fluorescens M3A detection, E. coli O157:H7 PhiV10nluc lysogen detection, and a luminescence-based detection of E. coli O157:H7 in ground meat using the engineered luminescent-based reporter phage PhiV10nluc, were conducted. In the same experimental setting, our previously developed smartphone-based luminometer called the bioluminescent-based analyte quantitation by smartphone and a conventional photomultiplier tube-based benchtop luminometer were used to compare detection levels and applicability for supporting luminescent phage-based pathogen detection. Results showed that the SiPM provides better performance in terms of time to detection and SNR and could be used as the light detection component of the PhiV10nluc phage-based detection format.Funding Information
- Agricultural Research Service (59-8072-6-001)
- National Institute of Food and Agriculture (1004830)
This publication has 31 references indexed in Scilit:
- SiPM as miniaturised optical biosensor for DNA-microarray applicationsSensing and Bio-Sensing Research, 2015
- Real-time monitoring of bioaerosols via cell-lysis by air ion and ATP bioluminescence detectionBiosensors and Bioelectronics, 2014
- Field-deployable whole-cell bioluminescent biosensors: so near and yet so farAnalytical and Bioanalytical Chemistry, 2013
- Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone SubstrateACS Chemical Biology, 2012
- Bioluminescence in analytical chemistry and in vivo imagingTrAC Trends in Analytical Chemistry, 2009
- A Genetically Encoded Bioluminescent Indicator for the Sodium Channel Activity in Living CellsJournal of the American Chemical Society, 2009
- Modeling and measurement of a whole-cell bioluminescent biosensor based on a single photon avalanche diodeBiosensors and Bioelectronics, 2008
- Status report on silicon photomultiplier development and its applicationsNuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2006
- Silicon photomultiplier and its possible applicationsNuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2003
- Bioluminescent whole cell optical fiber sensor to genotoxicants: system optimizationSensors and Actuators B: Chemical, 2001