Novel contribution in online sample cleanup for quantitative determination of Levetiracetam in human plasma by LC-Tandem mass spectrometry
Open Access
- 30 June 2016
- journal article
- Published by European Journal of Chemistry in European Journal of Chemistry
- Vol. 7 (2), 238-242
- https://doi.org/10.5155/eurjchem.7.2.238-242.1433
Abstract
The aim of this work was to develop and validate a simple, sensitive and rapid method for the quantitation of levetiracetam (LEV) in plasma using LC-Tandem mass spectrometry. Plasma samples were prepared by simple protein precipitation using acetonitrile; atenolol was used as internal standard (IS). Chromatographic separation was done on Luna 5 µm C18(2) (Phenomenex) 50 × 2.0 mm using gradient flow using solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile: water, 95:5, v:v). The positive-ion mass spectrometric detection method utilized electrospray ionization and the multiple reaction monitoring (MRM) mode. The MRM ion transitions were170.9 → 140.8 and 267.3 → 145.1 for levetiracetam and atenolol, respectively. The retention time of levetiracetam and atenolol is 3.24 and 2.96 min, respectively. The total run time was 5 min. The assay was validated over a concentration range from 1 to 100 µg/mL. The method was robust (minimal matrix effect), sensitive (LOQ, 1 µg/mL) metabolites and reproducible (The precision and accuracy for both intra- and inter-day were acceptable <15%). The method can be done on traditional LC-MS equipment. The method was effectively applied to single case study receiving toxic dose of LEV.Keywords
This publication has 18 references indexed in Scilit:
- Levetiracetam: A Review of its use in the treatment of epilepsyInternational Journal of Medicine and Biomedical Research, 2013
- Antiepileptic Drug Interactions - Principles and Clinical ImplicationsCurrent Neuropharmacology, 2010
- Simultaneous determination of Levetiracetam and its acid metabolite (ucb L057) in serum/plasma by liquid chromatography tandem mass spectrometryClinical Biochemistry, 2010
- Therapeutic Drug Monitoring of Levetiracetam by High-Performance Liquid Chromatography With Photodiode Array Ultraviolet Detection: Preliminary Observations on Correlation Between Plasma Concentration and Clinical Response in Patients With Refractory EpilepsyTherapeutic Drug Monitoring, 2007
- Drug Monitoring and Toxicology: A Procedure for the Monitoring of Levetiracetam and Zonisamide by HPLC-UVJournal of Analytical Toxicology, 2006
- Determination of levetiracetam in human plasma with minimal sample pretreatmentJournal of Chromatography B, 2005
- High‐performance liquid chromatographic determination of Levetiracetam in human plasma: comparison of different sample clean‐up proceduresBiomedical Chromatography, 2003
- Enantioselective analysis of levetiracetam and its enantiomer R-α-ethyl-2-oxo-pyrrolidine acetamide using gas chromatography and ion trap mass spectrometric detectionJournal of Chromatography B: Biomedical Sciences and Applications, 2000
- The new generation of antiepileptic drugs: advantages and disadvantagesBritish Journal of Clinical Pharmacology, 1996
- High-performance liquid chromatographic and megabore gas—liquid chromatographic determination of levetiracetam (ucb L059) in human serum after solid-phase extractionJournal of Chromatography B: Biomedical Sciences and Applications, 1994