Monitoring and Controlling the Dissolved Oxygen (DO) Concentration within the High Aspect Ratio Vessel (HARV)
- 5 September 2008
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 19 (4), 1335-1341
- https://doi.org/10.1021/bp0257574
Abstract
A probe-type oxygen sensor was developed utilizing a radioluminescent (RL)-based light source and a ruthenium-based sensing chemistry for monitoring the dissolved oxygen (DO) concentration in a modified version of the NASA-designed high aspect ratio vessel (HARV), a batch rotating wall vessel. This sensor provided the means to monitor the DO concentration in the HARV without influencing the flow pattern, thereby retaining the low shear HARV environment conducive to the formation of 3-dimensional cell aggregates. This sensor lost significant signal as a result of exposure to the first three autoclave cycles, but only minimal change in signal was observed following exposure to subsequent autoclave cycles. A new calibration model requiring only one fitted parameter was developed that accurately fit data over the entire range from 0% to 100% oxygen saturation. The ability for DO concentration control within the vessel was demonstrated by using this sensor to monitor the DO concentration inside the HARV.Keywords
This publication has 13 references indexed in Scilit:
- Continuous Oxygen Monitoring of Mammalian Cell Growth on Space Shuttle Mission STS-93 with a Novel Radioluminescent Oxygen SensorApplied Biochemistry and Biotechnology, 2003
- Radioluminescent sources for optical chemical sensorsPure and Applied Chemistry, 1999
- Steam‐Sterilizable, Fluorescence Lifetime‐Based Sensing Film for Dissolved Carbon DioxideBiotechnology Progress, 1998
- Radioluminescent Light Source for Optical Oxygen SensorsAnalytical Chemistry, 1997
- Oxygen-Sensitive Luminescent Materials Based on Silicone-Soluble Ruthenium Diimine ComplexesAnalytical Chemistry, 1995
- Modeling of Luminescence Quenching-Based Sensors: Comparison of Multisite and Nonlinear Gas Solubility ModelsAnalytical Chemistry, 1995
- Luminescence Quenching Behavior of an Oxygen Sensor Based on a Ru(II) Complex Dissolved in PolystyreneAnalytical Chemistry, 1995
- Phase fluorometric sterilizable optical oxygen sensorBiotechnology & Bioengineering, 1994
- Design of luminescence-based temperature sensorsPublished by SPIE-Intl Soc Optical Eng ,1993
- Temperature dependence of the photophysical and photochemical properties of the tris(2,2'-bipyridyl)ruthenium(II) ion in aqueous solutionJournal of the American Chemical Society, 1976