The present investigation was conducted to study the spectrum of mycoflora associated with phylloplane and rhizosphere of Aloe vera and to assess their effect on plant growth parameters and antagonistic activity against the C. gloeosporioides causing black spot disease in Aloe vera. During the study total 15 mycoflora belonging to ten genera were isolated from Aloe vera plant by leaf washing technique from phylloplane and serial dilution from rhizosphere soil. Among these, fungal species belonging to the genera of Aspergillus, Trichoderma and Penicillium was found to be more abundant. In course of study some of mycoflora associated with phylloplane and rhizosphere of Aloe vera were found to exert plant growth promoting effect and also exhibited strong antagonistic activity against Colletotrichum gloeosporioides.