Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition
Open Access
- 12 October 2019
- Vol. 11 (10), 2431
- https://doi.org/10.3390/nu11102431
Abstract
Fructooligosaccharides (FOS) are considered prebiotics and have been proven to selectively promote the growth of Bifidobacterium in the gut. This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice. Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3–V4 region of 16S rRNA and groEL gene, respectively. After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice. At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group. Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents. Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%). These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon. Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.Funding Information
- National Natural Science Foundation of China (31972086, 31601453, 31801530)
This publication has 38 references indexed in Scilit:
- Carbohydrate metabolism in BifidobacteriaGenes & Nutrition, 2011
- Metagenomic biomarker discovery and explanationGenome Biology, 2011
- Mucin-bacterial interactions in the human oral cavity and digestive tractGut Microbes, 2010
- Prebiotic oligosaccharides change the concentrations of short-chain fatty acids and the microbial population of mouse bowelJournal of Zhejiang University-SCIENCE B, 2009
- Mucins in the mucosal barrier to infectionMucosal Immunology, 2008
- Diversity of the Human Intestinal Microbial FloraScience, 2005
- Metabolization of β-(2,6)-linked fructose-oligosaccharides by different bifidobacteriaFEMS Microbiology Letters, 2000
- Metabolization of β-(2,6)-linked fructose-oligosaccharides by different bifidobacteriaFEMS Microbiology Letters, 2000
- Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestineJournal of Applied Bacteriology, 1993
- INDIGENOUS, NORMAL, AND AUTOCHTHONOUS FLORA OF THE GASTROINTESTINAL TRACTThe Journal of Experimental Medicine, 1965