Lipid Alterations during Zebrafish Embryogenesis Revealed by Dynamic Mass Spectrometry Profiling with C=C Specificity
- 18 October 2019
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Society for Mass Spectrometry
- Vol. 30 (12), 2646-2654
- https://doi.org/10.1007/s13361-019-02334-z
Abstract
Lipids exert substantial influences on vertebrate embryogenesis, but their metabolic dynamics at detailed structural levels remains elusive, primarily owing to the lack of a tool capable of resolving their huge structural diversity. Herein, we present the first large-scale and spatiotemporal monitoring of unsaturated lipids with C=C specificity in single developing zebrafish embryos enabled by photochemical derivatization and tandem mass spectrometry (MS). The lipid isomer composition was found extremely stable in yolk throughout embryogenesis, while notable differences in ratios of C=C location (e.g., PC 16:0_16:1 (7) vs. 16:0_16:1 (9)) and fatty acyl composition isomers (e.g., PC 16:1_18:1 vs. 16:0_18:2) were unveiled between blastomeres and yolk from zygote to 4 h post fertilization (hpf). From 24 hpf onwards, lipid isomer compositions in embryo head and tail evolved distinctively with development, suggesting a meticulously regulated lipid remodeling essential for cell division and differentiation. This work has laid the foundation for functional studies of structurally defined lipids in vertebrate embryology.Keywords
Funding Information
- National Natural Science Foundation of China (21705091, 21621003 and 21727813)
- Ministry of Science and Technology of the People's Republic of China (2016YFF0100301)
This publication has 53 references indexed in Scilit:
- KCTD13 is a major driver of mirrored neuroanatomical phenotypes of the 16p11.2 copy number variantNature, 2012
- Mutations in ISPD cause Walker-Warburg syndrome and defective glycosylation of α-dystroglycanNature Genetics, 2012
- Host Genotype-Specific Therapies Can Optimize the Inflammatory Response to Mycobacterial InfectionsCell, 2012
- Zebrafish as a model to study cardiac development and human cardiac diseaseCardiovascular Research, 2011
- Zebrafish Lipid Metabolism: From Mediating Early Patterning to the Metabolism of Dietary Fat and CholesterolMethods in Cell Biology, 2011
- A general introduction to the biochemistry of mitochondrial fatty acid β‐oxidationJournal of Inherited Metabolic Disease, 2010
- Analysis of the Zebrafish Proteome during Embryonic DevelopmentMolecular & Cellular Proteomics, 2008
- Membrane lipids: where they are and how they behaveNature Reviews Molecular Cell Biology, 2008
- Stages of embryonic development of the zebrafishDevelopmental Dynamics, 1995
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959