Effect of Pollyallylamine on Alcoholdehydrogenase Structure and Activity
Open Access
- 6 April 2020
- Vol. 12 (4), 832
- https://doi.org/10.3390/polym12040832
Abstract
In this article, the effect of polyallylamine (PAA) on the structure and catalytic characteristics of alcohol dehydrogenase (ADH) was studied. For this research, we used methods of stationary kinetics and fluorescence spectroscopy. It has been shown that PAA non-competitively inhibits ADH activity while preserving its quaternary structure. It was established that 0.1 M ammonium sulfate removes the inhibitory effect of PAA on ADH, which is explained by the binding of sulfate anion (NH4)2SO4 with polyallylamine amino groups. As a result, the rigidity of the polymer chain increases and the ability to bind to the active loop of the enzyme increases. It is also shown that sodium chloride removes the inhibitory effect of PAA on ADH due to an electrostatic screening of the enzyme from polyelectrolyte. The method of encapsulating ADH in polyelectrolyte microcapsules was adapted to the structure and properties of the enzyme molecule. It was found that the best for ADH is its encapsulation by adsorption into microcapsules already formed on CaCO3 particles. It was shown that the affinity constant of encapsulated alcohol dehydrogenase to the substrate is 1.7 times lower than that of the native enzyme. When studying the affinity constant of ADH in a complex with PAA to ethanol, the effect of noncompetitive inhibition of the enzyme by polyelectrolyte was observed.This publication has 28 references indexed in Scilit:
- Incorporation of proteins into polyelectrolyte microcapsules by coprecipitation and adsorptionRussian Journal of Bioorganic Chemistry, 2013
- Protein Purification by Polyelectrolyte Coacervation: Influence of Protein Charge Anisotropy on SelectivityBiomacromolecules, 2011
- Polyelectrolyte–protein complexation driven by charge regulationSoft Matter, 2009
- Enzyme-polyelectrolyte complex: Salt effects on the reaction of urease with polyallylamineRussian Journal of Physical Chemistry A, 2009
- Effect of Polyelectrolyte Structure on Protein−Polyelectrolyte Coacervates: Coacervates of Bovine Serum Albumin with Poly(diallyldimethylammonium chloride) versus ChitosanBiomacromolecules, 2007
- Protein Encapsulation via Porous CaCO3 Microparticles TemplatingBiomacromolecules, 2004
- Preparation and Organization of Nanoscale Polyelectrolyte‐Coated Gold NanoparticlesAdvanced Functional Materials, 2003
- Layer-by-Layer Engineering of Biocompatible, Decomposable Core−Shell StructuresBiomacromolecules, 2003
- Novel Hollow Polymer Shells by Colloid-Templated Assembly of PolyelectrolytesAngewandte Chemie, 1998
- Enzymes as reagents in clinical chemistryPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1983