A Model of Hormonal Regulation of Stamen Abortion during Pre-Meiosis of Litsea cubeba
Open Access
- 31 December 2019
- Vol. 11 (1), 48
- https://doi.org/10.3390/genes11010048
Abstract
Litsea cubeba (Lour.) Pers., a popular essential oil plant, is a dioecious species with degenerative sexual organs in both male and female individuals. Yet, the mechanism of degenerative organs development in male and female flowers is poorly understood. Here, we analyzed the morphological characters of degenerative organ development by morphological and histological observations, and determined the critical stage of abortion that occurs at pre-meiosis in male and female flowers. We also conducted RNA sequencing (RNA-seq) to understand the genetic basis of stamen abortion in female flowers. The differentially expressed genes (DEGs) were identified during the staminode development in female flowers; functional enrichment analysis revealed some important biological pathways involved the regulation of stamen abortion, including plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis and monoterpenoid biosynthesis. Furthermore, 15 DEGs involved in the hormone pathways were found to regulate stamen development. By HPLC-MS/MS analysis, there were a salicylic acid (SA) content peak and the gibberellin (GA) content lowest point in the abortion processes in female flowers, suggesting a vital function of hormonal processes. Co-expression network analysis further identified several hub genes that potentially played significant roles in the stamen abortion of L. cubeba. Taken together, we proposed a model involved in plant hormones pathways underlying stamen abortion during pre-meiosis in female flowers of L. cubeba.Funding Information
- The National Natural Science Foundation of China (31700523)
This publication has 45 references indexed in Scilit:
- AUXIN RESPONSE FACTOR17 Is Essential for Pollen Wall Pattern Formation in ArabidopsisPlant Physiology, 2013
- Full-length transcriptome assembly from RNA-Seq data without a reference genomeNature Biotechnology, 2011
- Arabidopsis Basic Leucine-Zipper Transcription Factors TGA9 and TGA10 Interact with Floral Glutaredoxins ROXY1 and ROXY2 and Are Redundantly Required for Anther DevelopmentPlant Physiology, 2010
- Functional analysis of B and C class floral organ genes in spinach demonstrates their role in sexual dimorphismBMC Plant Biology, 2010
- edgeR: a Bioconductor package for differential expression analysis of digital gene expression dataBioinformatics, 2009
- WGCNA: an R package for weighted correlation network analysisBMC Bioinformatics, 2008
- Mapping and quantifying mammalian transcriptomes by RNA-SeqNature Methods, 2008
- Global Identification of DELLA Target Genes during Arabidopsis Flower DevelopmentPlant Physiology, 2008
- The SUMO E3 ligase, AtSIZ1, regulates flowering by controlling a salicylic acid‐mediated floral promotion pathway and through affects on FLC chromatin structureThe Plant Journal, 2007
- Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction NetworksGenome Research, 2003