First detection of carbapenem resistance in Enterobacteriaceae isolates isolated from dairy cows’ mastitis infection in Türkiye

Abstract

With this study, carbapenem resistance genes were declareted for the first time in Enterobacteriaceae isolates isolated from animals in Turkey. On bacteriological examination of milk samples, E. coli was isolated in 14 (6.60%), Klebsiella oxytoca was isolated in 3 (1.41%) and Klebsiella pneumonia was isolated in 2 (0.94%) a total of 212 samples. At least 2 E. coli isolates were detected resistant to all of the active substances used in the test except for Penicillin+Framycetin. All the isolates were detected susceptible to Penicillin+Framycetin. The highest resistance in K. oxytoca isolates was found to be against cefotaxime, whereas, the highest resistance detected in K. oxytoca isolates against sulphametaxazole/trimethoprim. According to the PCR results of blaCTX-M, blaTEM and blaSHV genes, blaCTX-M gene was detected in 1 K. oxytoca isolates with positive ESBL and 4 E. coli isolates. According to the PCR results of carbapenemase and colistin resistance genes, IMP gene were detected in 4 E.coli, 1 K. oxytoca and 1 K. pneumonia isolates. OXA-48-like gene were detected in 2 E. coli isolates. In these 2 E. coli isolates also has IMP gene. While NDM and mcr-1 genes were detected 2 E. coli, NDM gene was detected in 1 E. coli isolate. As a result of identification of ESBL and carbapenem resistance in the species in Enterobacteriaceae family becoming prevalent and increasing, especially among E. coli isolates. Also, identification of multiple antibiotic resistance of active substance in the isolates indicated that antibiotic resistance also spread rapidly and increased.