Predictive phytotoxic value of water-soluble allelochemicals in plant extracts for choosing a cover crop or mulch for specific weed control

Abstract

Cover crops and mulches have become an alternative for soil management in vineyards due to the agronomic, environmental, and economic advantages, especially the possibility of weed control. Implicitly to this objective lies the idea of assessing the potential herbicide effect of the allelochemicals released by different cover crop and mulch species. With this objective, the present work evaluated the phytotoxic effects of 12 aqueous extracts of selected species with potential use as a cover crop or mulch: a Bromus species mixture (B. hordeaceus L. and B. rubens L.), Festuca arundinacea Schreb., Hordeum murinum L., H. vulgare L., Vulpia ciliata Dumort., Medicago rugosa Desr., M. sativa L., Trifolium subterraneum L., T. incarnatum L., Phacelia tanacetifolia Benth., Sinapis alba L., and Pinus sylvestris L.,on the germination and early growth of three troublesome weeds (Conyza bonariensis (L.) Cronquist, Aster squamatus (Spreng.) Hieron, and Bassia scoparia (L.) A. J.). The different in vitro bioassays showed that aqueous extracts of some species significantly inhibited or reduced germination and root and shoot growth of the target weed species in a dose-response manner. Germination of A. squamatus and C. bonariensis was reduced by 100-80% by the different extracts applied at 50% concentration, and completely blocked at 100% concentration, except for M rugosa extract to which both species showed less sensitivity. Root elongation of A. squamatus was absolutely inhibited under every extract and concentration, whereas C. bonariensis root growth showed only some tolerance to the crude extracts of F. arundinaceaand P. sylvestris. Bassia scoparia was relatively tolerant to the aqueous plant extracts, except for T. subterraneum crude extract, which reduced total germination by 80%; otherwise, B. scoparia showed higher general sensitivity of shoot growth than the other two weed species. The chemical profiles of phenolic compounds of the aqueous extracts were obtained and identified by HPLC-DAD, the phenolic profiles of H. murinum, V. ciliata, and M. rugosa being reported in this work for the first time. Using stepwise regression, the influence of certain phenolic compounds from the aqueous extracts on the germination and early growth of weeds was predicted. Among other significant compounds, the flavonoid naringenin identified in T. subterraneumaqueous extract at 8.09 µg·mL^-1 was predicted to underlie its specific phytotoxicity on B. scoparia germination. These results support the use of cover crops and mulches in weed management and can help to select the most suitable species to adopt according to the target weed species. Highlights