Low membrane fluidity triggers lipid phase separation and protein segregation in living bacteria
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Open Access
- 17 January 2022
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 41 (5), e109800
- https://doi.org/10.15252/embj.2021109800
Abstract
All living organisms adapt their membrane lipid composition in response to changes in their environment or diet. These conserved membrane-adaptive processes have been studied extensively. However, key concepts of membrane biology linked to regulation of lipid composition including homeoviscous adaptation maintaining stable levels of membrane fluidity, and gel-fluid phase separation resulting in domain formation, heavily rely upon in vitro studies with model membranes or lipid extracts. Using the bacterial model organisms Escherichia coli and Bacillus subtilis, we now show that inadequate in vivo membrane fluidity interferes with essential complex cellular processes including cytokinesis, envelope expansion, chromosome replication/segregation and maintenance of membrane potential. Furthermore, we demonstrate that very low membrane fluidity is indeed capable of triggering large-scale lipid phase separation and protein segregation in intact, protein-crowded membranes of living cells; a process that coincides with the minimal level of fluidity capable of supporting growth. Importantly, the in vivo lipid phase separation is not associated with a breakdown of the membrane diffusion barrier function, thus explaining why the phase separation process induced by low fluidity is biologically reversible.Keywords
Funding Information
- Biotechnology and Biological Sciences Research Council (BB/S00257X/1, BB/M011186/1)
- Medical Research Foundation (MR/N013840/1)
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