β-glucan–dependent shuttling of conidia from neutrophils to macrophages occurs during fungal infection establishment
Open Access
- 4 September 2019
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLoS Biology
- Vol. 17 (9), e3000113
- https://doi.org/10.1371/journal.pbio.3000113
Abstract
The initial host response to fungal pathogen invasion is critical to infection establishment and outcome. However, the diversity of leukocyte–pathogen interactions is only recently being appreciated. We describe a new form of interleukocyte conidial exchange called “shuttling.” In Talaromyces marneffei and Aspergillus fumigatus zebrafish in vivo infections, live imaging demonstrated conidia initially phagocytosed by neutrophils were transferred to macrophages. Shuttling is unidirectional, not a chance event, and involves alterations of phagocyte mobility, intercellular tethering, and phagosome transfer. Shuttling kinetics were fungal-species–specific, implicating a fungal determinant. β-glucan serves as a fungal-derived signal sufficient for shuttling. Murine phagocytes also shuttled in vitro. The impact of shuttling for microbiological outcomes of in vivo infections is difficult to specifically assess experimentally, but for these two pathogens, shuttling augments initial conidial redistribution away from fungicidal neutrophils into the favorable macrophage intracellular niche. Shuttling is a frequent host–pathogen interaction contributing to fungal infection establishment patterns.Funding Information
- Department of Health, Australian Government
- National Health and Medical Research Council (461208)
- National Health and Medical Research Council (637394)
- National Health and Medical Research Council (1044754)
- National Health and Medical Research Council (1069284)
- National Health and Medical Research Council (637367)
- Australian Research Council (DP1094854)
- National Institutes of Health (5RO1HL124209)
- Australian Postgraduate Award
- Australian Postgraduate Award
- Walter and Eliza Hall Institute Edith Moffatt Scholarship
- Walter and Eliza Hall Institute Edith Moffatt Scholarship
- Monash University Bridging Postdoctoral Fellowship
- Monash Graduate Scholarship
- Monash International Postgraduate Research Fellowship
- Monash Postgraduate Publication Award
- National Health and Medical Research Council
- National Institutes of Health (EB002503)
- Intellectual and Developmental Disabilities Research Center (1U54HD0902565)
- National Institutes of Health (NS047101)
- State Government of Victoria
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