Effect of caffeine in chocolate (Theobroma cacao) on the alveolar bone mineral density in guinea pigs (Cavia cobaya) with orthodontic tooth movement
Open Access
- 15 September 2020
- journal article
- Published by Universitas Airlangga in Dental Journal (Majalah Kedokteran Gigi)
- Vol. 53 (3), 164-169
- https://doi.org/10.20473/j.djmkg.v53.i3.p164-169
Abstract
Background: The benefits of chocolate have attracted significant attention from clinicians, especially the active compound of caffeine on bone metabolism. The bone density significantly affected the rate of tooth movement. Purpose: This study aims to analyse the effect of the dose and the duration of caffeine consumption in chocolate on alveolar bone mineral density in orthodontic tooth movement. Methods: Forty-eight male guinea pigs (Cavia cobaya) aged between 3-4 months and weighing 300-350 grams were divided into four groups (group A control, group B caffeine dose of 2.3 mg, group C caffeine dose of 3.45 mg, and group D caffeine dose of 4.6 mg). An open coil spring was applied to the mandibular inter-incisor with an orthodontic force of 35 grams. Guinea pigs were sacrificed using lethal doses of anaesthetics on days 0, 1, 7, and 14 after an orthodontic appliance installation. Mandibular alveolar bone mineral density in compression sites was analysed with an atomic absorption spectrophotometer (AAS). Experiment data results were analysed using two-way ANOVA with a 95% degree of confidence. Results: Caffeine consumption with a dose of 4.6 mg on day 7 had the lowest alveolar bone mineral density and the highest was at a dose of 2.3 mg on day 14, but there were no differences between the dose groups, the duration groups and interactions between both of them (p>0.05). Conclusion: The consumption of caffeine in chocolate did not decrease the bone mineral density in the compression site of orthodontic tooth movement.Keywords
This publication has 21 references indexed in Scilit:
- Bone-mineral density: clinical significance, methods of quantification and forensic applicationsResearch and Reports in Forensic Medical Science, 2019
- A Novel Method to Quantify Longitudinal Orthodontic Bone Changes with In Vivo Micro-CT DataJournal of Healthcare Engineering, 2018
- The Safety of Ingested Caffeine: A Comprehensive ReviewAmerican Journal of Translational Research, 2017
- Calcium and Bone Metabolism IndicesPublished by Elsevier BV ,2017
- Signaling Pathways in Osteoclast DifferentiationChonnam Medical Journal, 2016
- ANALISIS KANDUNGAN KAFEIN PADA KOPI DI DESA SESAOT NARMADA MENGGUNAKAN SPEKTROFOTOMETRI UV-VISJurnal Kimia, 2016
- Identifikasi Perawatan Ortodontik Spesialistik dan UmumMajalah Kedokteran Gigi Indonesia, 2013
- Drinking coffee may help accelerate orthodontic tooth movementDental Hypotheses, 2012
- Caffeine enhances osteoclast differentiation from bone marrow hematopoietic cells and reduces bone mineral density in growing ratsJournal of Orthopaedic Research, 2011
- Bone quality associated with daily intake of coffee: a biochemical, radiographic and histometric studyBrazilian Dental Journal, 2010