A naturally occurring point mutation in the hyaluronidase gene (hysA1) of Staphylococcus aureus UAMS-1 results in reduced enzymatic activity
- 1 January 2022
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 68 (1), 31-43
- https://doi.org/10.1139/cjm-2021-0110
Abstract
Hyaluronic acid is a high molecular weight polysaccharide that is widely distributed in animal tissues. Bacterial hyaluronidases degrade hyaluronic acid as secreted enzymes and have been shown to contribute to infection. Staphylococcus aureus UAMS-1 is a clinical isolate that codes for two hyaluronidases (hysA1 and hysA2). Previous research has shown the presence of a full-length HysA1 protein from the S. aureus UAMS-1 strain with no evidence of enzymatic activity. A single base change resulting in an E480G amino acid change was identified in the S. aureus UAMS-1 hysA1 gene when compared to the S. aureus Sanger 252 hysA1 gene. A plasmid copy of the S. aureus Sanger 252 hysA1 gene transduced into a hysA2 deletion mutant strain of S. aureus UAMS-1 restored near wild type levels of enzymatic activity. Homology modeling and structural analysis suggested that Glu-480 in the HysA1 is critically responsible for maintaining the structural and functional ensemble of the catalytic and tunnel-forming residues, which are essential for enzyme activity. A high degree of relatedness among several Gram-positive bacterial hyaluronidases indicate the loss of enzymatic activity of HysA1 in the S. aureus UAMS-1 strain is most likely caused by the mutation identified in our study.Keywords
This publication has 67 references indexed in Scilit:
- Colonization, pathogenicity, host susceptibility, and therapeutics for Staphylococcus aureus: what is the clinical relevance?Seminars in Immunopathology, 2011
- Protein structure analysis of mutations causing inheritable diseases. An e-Science approach with life scientist friendly interfacesBMC Bioinformatics, 2010
- The RCSB Protein Data Bank: redesigned web site and web servicesNucleic Acids Research, 2010
- Community-associated meticillin-resistant Staphylococcus aureusThe Lancet, 2010
- Waves of resistance: Staphylococcus aureus in the antibiotic eraNature Reviews Microbiology, 2009
- Genotypic and Phenotypic Assessment of Hyaluronidase among Type Strains of a Select Group of Staphylococcal SpeciesInternational Journal of Microbiology, 2009
- Relative Quantitative Comparisons of the Extracellular Protein Profiles ofStaphylococcus aureusUAMS-1 and ItssarA,agr, andsarA agrRegulatory Mutants Using One-Dimensional Polyacrylamide Gel Electrophoresis and Nanocapillary Liquid Chromatography Coupled with Tandem Mass SpectrometryJournal of Bacteriology, 2008
- SarA ofStaphylococcus aureusBinds to thesarAPromoter To Regulate Gene ExpressionJournal of Bacteriology, 2008
- CUPSAT: prediction of protein stability upon point mutationsNucleic Acids Research, 2006
- Staphylococcus aureus–associated Skin and Soft Tissue Infections in Ambulatory CareEmerging Infectious Diseases, 2006