Background: Pancreatic cancer is a common disease of digestive cancers. The tumor progresses rapidly and malignantly, showed a low 5-year survival rate. However, when diagnosed and treated early, the 5-year survival rate of pancreatic cancer improves significantly. Endoscopic ultrasound (EUS) has the advantage that probe can optimal access to the pancreas and surrounding organs, therefore, diagnose pancreatic tumors can be made even when the tumor is small (< 2 cm). Furthermore, Endoscopic Ultrasound- Guided Fine needle aspiration (EUS-FNA) can determine the exact histology of the tumor. Objectives: 1. Investigate some clinical, biological and endoscopic ultrasound characteristics in patients with pancreatic cancer. 2. Evaluate the relationship between endoscopic ultrasound images with clinical and biological characteristics and compare the results between endoscopic ultrasound with computer tomography. Materials and methods: Descriptive cross-sectional studies in 58 patients with pancreatic cancer had endoscopic ultrasound. Results: In 58 patients with pancreatic cancer, we recorded that patients with a history of smoking accounted for 32.8%, and drinking alcohol 31%. Abdominal pain was 89.7%, 44.8% of patients show anorexia, 37.9% have weight loss. 69% of patients had CA 19-9 > 37 UI/ml. EUS found a patient with pancreatic tumor < 2 cm, but CT scan could not detect. Tumors in pancreatic head accounted for 60.3%; in the body 24.1%, 96.6% of pancreatic cancer have a hypoechoic structure, heterogeneous ultrasound. There was a significant relation between pancreatic head tumor and other part with jaundice (p = 0.013). Compatibility in diagnosis of vascular invasion between EUS and CT scan was average (K = 0.483). Conclusion: Smoking and alcohol consumption accounted for the highest proportion, abdominal pain was the most common clinical symptom. Endoscopic ultrasound can diagnose small pancreatic tumors that CT scan can not detect. Key words: Pancreatic cancer, endoscopic ultrasound, clinical characteristics, bio-marker