Q fever is an emerging worldwide zoonotic disease caused by a bacterium Coxiella burnetii. Detection of Q fever antibodies among humans and animals was conducted using sera from human donors and sera from three different species of animals obtained from Catarman, Northern Samar, Los Baños, Laguna, and General Santos City, South Cotabato, Philippines. The Complement Fixation Test (CFT) was used to detect antibodies in sera samples. The result of the study showed that Q fever antibodies in humans were found to be 37% (22/60) while the sampled animals have 43 % (77/180). Among the animals, the highest occurrence of antibodies was observed in goats with 70% (42/60), followed by 40% among cattle (24/60) and the least 18% were from carabaos (11/60). According to sampling sites, 20% (12/60) of antibody-positive humans were from Los Baños, Laguna, some 17% (10/60) came from General Santos City and none from Catarman, Northern Samar. Among the goats examined, a higher percentage of Q fever antibodies were obtained from those in Los Baños with 30% (18/60) occurrence, and from General Santos City with 25% (15/60) occurrence. Likewise, an equal occurrence rates of Q fever antibodies among cattle came from Los Baños, Laguna and General Santos City each with 20% (12/60) seropositivity, respectively. Among the carabaos, 18% (11/60) was in General Santos City and 8% (5/60) in Catarman. Based on these findings, it could be inferred that Q fever infection exists among humans, goats, cattle, and carabaos in the Philippines.